摘要
利用阿利新蓝(AB,pH=2.6)-过碘酸雪夫氏试剂(PAS)染色,对鲇(Silurus asotus)皮肤和鳃部粘液细胞进行了分类、分布和分泌研究。结果显示:结合染色结果、细胞形态和粘液细胞分布特点,鲇皮肤和鳃部粘液细胞可分为Ⅰ型、Ⅱ型、Ⅲ型、Ⅳ型和Ⅴ型5种类型;皮肤中有Ⅰ型和Ⅳ型2种粘液细胞分布,鳃部有Ⅰ型、Ⅱ型、Ⅲ型和Ⅴ型4种粘液细胞分布;腹部皮肤粘液细胞密度最大,平均密度为每平方毫米1083个,其次为颌部皮肤,平均密度为每平方毫米802个,鳃丝部位密度最小,平均密度为每平方毫米208个;粘液细胞分泌方式有4种,Ⅰ型为全浆分泌,Ⅱ型为局部分泌,Ⅲ型为巨浆分泌,Ⅳ型为顶浆分泌。
The types,distribution and secretion of mucous cells stained with AB-PAS (pH AB = 2.6) in the skin and gill of Silurus asotus (body length 35 ± 10 cm,body weight 650 ± 150 g) were studied.The results showed that the mucous cells in the skin and gill of Silurus asotus could be classified into five types according to colorization with AB-PAS,the shape of the cells and distribution position,i.e.Type Ⅰ,Type Ⅱ,Type Ⅲ,Type Ⅳ and Type Ⅴ mucous cells;In the skin,there were types Ⅰand Ⅲ mucous cells,and in the gill,there were types Ⅰ,Ⅱ,Ⅲ and Ⅴ mucous cells;The density of mucous cells was the highest in the ventral skin,and the mean density was about 1 083 cells /mm^2.Next is in the sbmaxillary skin,and the mean density was about 802 cells /mm^2.The density of mucous cells was the lowest in the gill filament,and the mean density was about 208 cells /mm^2;The mucous cells had 4 kinds of secretion,the type Ⅰmucous cells had holocrine secretion,the type Ⅱ mucous cells had partial secretion,the type Ⅲ mucous had macroapocrine secretion,the type Ⅳ mucous cells had apocrine secretion.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第6期751-755,共5页
Journal of Shanghai Ocean University
基金
河南省重点科技攻关项目(82102130022)
关键词
鲇
粘液细胞
皮肤
鳃
Silurus asotus
mucous cell
skin
gill