仿刺参4种消化酶活力的周年变化

Changes of four digestive enzymes in sea cucumber (Apostichopus japonicus)

2004年11月至2005年10月每月中旬,在大连黑石礁海区采捕体长(21.8±2.3)cm(麻醉状态下),体质量(165.3±3.1)g的仿刺参(Apostichopus japonicus),测定消化道中蛋白酶、淀粉酶、褐藻酸酶和纤维素酶活性的周年变化。结果表明,1-5月间,消化管的最大长度可达体长的4.6～5.9倍,2月达全年的最大值;蛋白酶、淀粉酶和褐藻酸酶的活力随水温的升高而增加,4-5月达最大值;之后,消化管长/体长比逐渐下降,8-10月上旬,消化管长仅为体长的0.8～1.1倍,酶活力逐渐下降;10月中下旬,随着水温的下降,消化管长/体长比又快速增大,酶活力也逐渐回升。全年中,纤维素酶的活力一直很低,周年变化不明显。

Changes of protease,amylase,algalase and cellulase activities in alimentary canal were analyzed in sea cucumber （Apostichopus japonicus） weighing 165.3 ± 3.1 g collected monthly along Dalian coast from November 2004 to October 2005.The results showed that the ratio of digestive tract length to body length （RDTLTBL） was 4.6-5.9 from January to May,with the peak in February in the sea cucumbers.Meanwhile,the activities of protease,amylase,and algalase increased with water temperature rising,the maximum value in April and May.Afterwards,there was decrease in the （RDTLTBL） as water temperature rose,0.8-1.1 from August to early October.In the same period,the activities of protease,amylase,and algalase decreased gradually.While the water temperature decreased in middle and later October,the （RDTLTBL） and the activities of protease,amylase,and algalase increased rapidly.There was low and constant cellulase activity all of the year.