酶免分析法检测甲状腺双抗体在临床中的应用

ELISA double antibody assay of thyroid clinical application

目的:探讨抗甲状腺球蛋白抗体(Anti-Tg)和抗甲状腺过氧化物酶抗体(Anti-TPO)检测在临床上较高阳性率。方法:采用微粒子酶免分析法(MEIA)分析医院就诊疑似甲状腺疾病的患者225例,并与健康体检人群42例检测Anti-Tg和Anti-TPO检测阳性率进行对照。结果:医院就诊疑似甲状腺疾病的患者阳性率(Anti-Tg为40.9%、Anti-TPO为31.6%)明显高于健康体检人群阳性率(Anti-Tg为16.7%、Anti-TPO为14.3%),两组比较差异有统计学意义。结论:通过MEIA检测血清中Anti-Tg和Anti-TPO水平,与甲状腺疾病有密切关系,可作为甲状腺疾病的诊断指标。MEIA对Anti-Tg和Anti-TPO进行定量测定,其检测速度快、灵敏度高、重复性好,自动化程度高,具有良好的稳定性,检测效率高,能及时为临床上诊断、治疗甲状腺疾病提供实验依据,值得推广。

Objective：To explore the high positive rate of thyroglobulin antibody （Anti-Tg） and anti-thyroid peroxidase antibody （Anti-TPO） detection in clinic.Methods：The hospital examined 225 patients suspected of thyroid disease,using micro particle enzyme immunoassay （MEIA）,and compared it to the positive rate of 42 clients of health examination by AntiTg and Anti-TPO detection.Results：The positive rate of the patients suspected of thyroid disease （Anti-Tg was 40.9%,Anti-TPO was 31.6%） was apparently higher than the healthy clients （Anti-Tg was 16.7%,Anti-TPO was 14.3%）,and two groups of comparisons have obvious statistical differences.Conclusion：Closely relate to the thyroid disease,the Anti-Tg and Anti-TPO level in serum results from MEIA detection,can be applied as a diadynamic criteria.MEIA conducts quantitative determination to Anti-Tg and Anti-TPO,with high detection speed,sensitivity,repeatability,and high degree of automation,credible stability and efficiency.It can be provide timely experimental evidence for clinical diagnosis and treatment of thyroid disease,and is worth to be popularized.