期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

OmpC高表达与大肠埃希氏菌四环素耐药的关系 被引量：2

Study of the Relationship of OmpC Overexpression and Tetracycline Resistance in Escherichia coli
下载PDF
导出
摘要 大肠埃希氏菌(Escherichia coli)外膜蛋白OmpC与细菌耐药关系密切,但有关其高表达与大肠埃希氏菌耐药的关系研究尚不清楚.为深入探讨OmpC的耐药功能,将ompC克隆到pET-28a载体上进行诱导表达,采用MALDI-TOF质谱分析证明,重组OmpC能够在外膜上大量表达.以此高表达菌株进行四环素耐药试验发现,ompC-pET-28a-BL21的最低抑菌浓度(MIC)值是对照菌株pET-28a-BL21的64倍.研究结果从蛋白质水平进一步证明OmpC在大肠埃希氏菌耐药中的重要作用. Outer membrane protein（OmpC） was thought to related to tetracycline resistance in E.coli,but the relationship between the overexpression of OmpC and tetracycline resistance was poorly understood.In the present study,ompC was cloned to pET-28a and MALDI-TOF showed recombined OmpC was highly expressed in the outer membrane of ompC-pET-28a-BL21.The minimum inhibitory concentration（MIC） of tetracycline of ompC-pET-28a-BL21 was 192 μg/mL,64 times higher than pET-28a-BL21.These results confirmed the importance of OmpC in tetracycline resistance in E.coli at protein level directly.
作者 张丹凤 李惠 王三英
机构地区 厦门大学生命科学学院 中山大学生命科学学院
出处 《厦门大学学报（自然科学版）》 CAS CSCD 北大核心 2010年第6期858-862,共5页 Journal of Xiamen University：Natural Science
基金 国家自然科学基金资助项目(40976080)
关键词 OMPC 大肠埃希氏菌 耐药性 高表达 OmpC E.coli drug resistance overexpression
分类号 R378.21 [医药卫生—病原生物学]
  • 相关文献

参考文献17

  • 1Chopra I, Hawkey P M, Hintion M. Tetracyclines, molecular and clinical aspects [J]. J Antimicrob Chemother, 1992,29 (3) :245-277.
  • 2McDermott P F,Walker R D,White D G. Antimierobials: models of action and mechanisms of resistance[J]. Int J Toxicol,2003,22 (2) : 135-143.
  • 3Koebnik R,Locher K P, Gelder P V. Structure and function of bacterial outer membrane proteins: barrels in a nutshell[J]. Mol Microbiol, 2000 ; 37 (2) :239-253.
  • 4Schirmer T. General and specific porins from bacterial outer membranes[J]. J Struct Biol, 1998,121 (2):101-109.
  • 5Batchelor E, Walthers D, Kenney L J, et al. The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins OmpF and OmpC[J]. J Bacteriol, 2005,187 (16) :5723-5731.
  • 6Lin X M,Li H,Wang C,et al. Proteomic analysis of nalidixie acid resistance in Escherichia coli : identification and functional characterization of OM proteins[J]. J Proteome Res,2008,7(6) :2399-2405.
  • 7Li H,Lin X M, Wang S Y, et al. Identification and antibody therapeutic targeting of chloramphenicol-resistant outer membrane proteins in Escherichia coli[J]. J Proteome Res,2007,6(9):3628-3636.
  • 8Xu C X,Lin X M,Ren H X,et al. Analysis of outer membrane proteome of Escherichia coli related to resistance to ampicillin and tetracycline [J]. Proteomies, 2006, 6 (2): 462-473.
  • 9Zhang D F,Jiang B, Xiang Z M, et al. Functional characterization of altered outer membrane proteins for tetracycline resistance in Escherichia coli [J]. Int J Antimicrob Agents,2008,32(4) :315-319.
  • 10Sabri M Y,Zamri-Saad M, Mutalib A R,et al. Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep[J]. Vet Microbiol, 2000,73 (1) : 13-23.

二级参考文献14

  • 1Chopra I, Hawkey P M, Hinton M. Tetracyclines, molecular and clinical aspects [J]. J Antimicrob Chemother, 1992,29 (3) : 245-277.
  • 2Flakow S. Infectious multiple drug resistance [M]. Pion Limited, London : 1975 : 81 - 82.
  • 3Akiba T, Koyama K, Ishiki Y, et al. On the mechanism of the development of multiple-drug-resistant clones of Shigella [J]. Jpn J Microbiol,1960,4(4):219-227.
  • 4Watanabe T. Infective heredity of multiple drug resistance in bacteria [J]. Bacterlol Rev,1963,27(1) :87-115.
  • 5McDermott P F, Walker R D, White D G. Antimicrobials: models of action and mechanisms of resistance [J]. Int J Toxicol,2003,22(2) : 135-143.
  • 6Fralick J A. Evidence that TolC is required for functioning ofthe Mar/AcrAB efflux pump of Escherichia coli [J] . d Bacteriol, 1996,178(19) : 5803- 5805.
  • 7Yoshida K, Nakayama K, Kuru N, et al. MexAB-OprM specific efflux pump inhibitors in Pseudomonas aeruginosa. Part 5, Carbon-substituted analogues at the C-2 position [J]. Bioorg Med Chem,2006,14(6) : 1993-2004.
  • 8Xu C X, Lin X M, Ren H X, et al. Analysis of outer membrane proteome of Escherichia coli related to reslstance to ampicillin and tetracycline [J]. Proteomics,2006, 6(2):462-473.
  • 9Cristobal R E, Vincent P A, Salomon R A. Multidrug resistance pump AcrAB-TolC is required for high-level, Tet (A)-mediated tetracycline resistance in Escherlchia coli [J]. J Antlmicrob Chemother , 2006 , 58 (1) : 31- 36.
  • 10Peng X X, Zhang J Y, Wang S Y, et al. Immuno-capture PCR for Detection of Aeromonas hydrophila [J]. J Microbiol Methods,2002,49 (3) : 335-335.

同被引文献42

  • 1章跃陵,林伯坤,陈俊,胡忠,黄通旺,严芳.凡纳滨对虾血蓝蛋白的细菌凝集活性[J].中国水产科学,2006,13(6):1006-1011. 被引量：22
  • 2McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance [ J]. Clin Microbiol Rev, 1999, 12(1) : 147-179.
  • 3Tumah HN. Bacterial biocide resistance [ J ]. J Chemother, 2009, 21(1) : 5-15.
  • 4Russell AD. Similarities and differences in the responses of microorganisms to biocides[ J]. J Antimicrob Chemother, 2003, 52(5) : 750-763.
  • 5Tattawasart U, Maillard JY, Furr JR, et al. Outer membrane changes in Pseudomonas stutzeri resistant to chlorhexidine diacetate and cetylpyridinium chloride [ J ]. Int J Antimicrob Agents, 2000, 16(3): 233-238.
  • 6Chiu HC, Lin TL, Wang JT. Identification and characterization of an organic solvent tolerance gene in Helicobacter pylori [ J ]. Helicobacter, 2007, 12( 1 ) : 74-81.
  • 7Putrins M, Ilves H, Lilje L, et al. The impact of CoIRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria [J]. BMC Microbiol, 2010, 10:110-121.
  • 8Baba T, Ara T, Hasegawa M, et al. Construction of Escherichia coli K-12 in-frame, singlegene knockout mutants: the Keio collection[J]. Mol Syst Biol, 2006, 2 : 2006. 0008.
  • 9Li H, Wang BC, Xu WJ, et al. Identification and network of outer membrane proteins regulating streptomysin resistance in Escherichia coli[ J]. J Proteome Res, 2008, 7(9) : 4040-4049.
  • 10Wang Y. The function of OmpA in Escherichia coli[J]. Biochem Biophys Res Commun, 2002, 292 (2) : 396-401.

引证文献2

二级引证文献3

厦门大学学报（自然科学版）
2010年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部