毒理学领域的纳米颗粒分散方法的生物安全性研究

Nanoparticles dispersing method for toxicology study

目的筛选比较目前常用分散剂及其生物安全性效果,从而寻找适用于生物安全性研究的纳米颗粒分散方法。方法通过对纳米TiO_2分散液的纳米颗粒粒径分析、多分散指数测定、沉降速率研究,以及不同分散剂对细胞活力的影响,选择最佳的具有生物安全性的纳米颗粒分散方法。结果 2%Tween的分散粒径最小,25%的胎牛血清(FBS)随时间变化粒径变化较小,溶液体系最稳定;25%FBS制备的二氧化钛悬液的吸光度较高,其变化类似于2%牛血清白蛋白(BSA),该体系中沉降的二氧化钛颗粒少,分散较好;细胞活力影响,与普通培养基相比较2%Tween对细胞活性影响最大,25%FBS和2%BSA对细胞活性影响较轻。结论使用分散剂后有效抑制了TiO_2悬浮液的团聚现象。25%FBS和2%BSA分散效果较优,分散体系稳定随时问变化小,溶液稳定性好,生物安全性能佳,是较好的分散剂。

Objective To compare the selection of the dispersing agent used and to evaluate the biosafety and to established the optimal dispersing method for nanotoxicological study. Methods The different suspensions of TiO2 dispersion and cell viability, particle size, PdI, and absorbance were detected. After comparing their dispersion effect, the best method was selected. Results The results of particle size, polydispersity index （ PdI）, absorbance, and cell activity showed that 2% Tween 20 dispersion had the smallest particle size. 25% FBS dispersion was most stable for the high absorbanee, similar to the changes in 2% BSA. 2% Tween 20 decrease cell viability. 25% FBS and 2% BSA had less effect on cell activity. Conclusions Dispersants suppress the TiO2 agglomeration. 25% FBS and 2% BSA are better dispersants for the stable dispersion.