HPLC法检测柑橘中赤霉酸残留量的色谱条件

Chromatographic Conditions of the Determination of GA3 Residue in Citrus by HPLC

在确定了赤霉酸适宜检测波长的基础上,以保留时间、分离度、拖尾因子、谱图峰型、相对标准偏差等为指标,分别考察流动相甲醇与水的体积比、流动相流速及柱温对柑橘中赤霉酸检测结果的影响。结果显示,采用反相C18柱的HPLC法检测赤霉酸的适宜色谱条件为：柱温为35℃,流动相为体积比为40/60的甲醇-水溶液（pH4.0）,流速为1.0 mL/min,检测波长为210 nm。在此条件下,HPLC检测柑橘中赤霉酸含量的灵敏度高（最低检出限0.033 4μg,S/N=3）,准确度较好（平均加标回收率为86.0%~91.0%,n=5）,精密度较高（平均RSD为1.409 7%,n=5）。

Study the wavelength,ratio of methanol and water,flow rate and column temperature on the determination of GA3 residue in citrus by HPLC,by the index of retention time,resolution,tailing factor,peak,and RSD et al.The result showed the optimum chromatographic conditions of HPLC were： C18 column;column temperature 35 ℃;mobile phase： CH3OH/H2O=40/60（pH 4）;flow rate 1.0 mL/min;wavelength 210 nm.Under these experimental conditions,the method of determination of GA3 by HPLC is sensitive（the limit of detection： 0.033 4 μg,S/N=3,） accurate（the recovery of standard addition： 86.0 %-93.4 %,n=5）,precise（RSD： 1.409 7 %,n=5.）