食品中产志贺毒素大肠杆菌的多重PCR检测

A Multiplex PCR System for the Detection of Shiga Toxin-producing Escherichia Coli in Food

针对产志贺毒素大肠杆菌的stx2、wzy、hlyA的保守序列,设计特异性的引物,建立一种新的多重PCR检验方法。结果显示,该方法特异性强,扩增结果与各参考菌株基因型一致,并能良好的区分出O157菌株和非O157型产志贺毒素大肠杆菌。该方法能用于食品样品的检测和流行病学分离株的快速鉴定。

Specific primers were designed according to the conserved loci of stx2,wzy and hlyA genes of Shiga toxin-producing Escherichia coli（STEC）.A Multiplex PCR method was then developed.The result demonstrated that this method was highly specific.It detected the presence of the three target genes in a manner consistent with the known genotype of each reference strain.O157 and non-O157 STEC strains could also be well distinguished.The multiplex PCR developed in this study could be used for mass screening of food samples,and identification of clinical STEC isolates.