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牛乳中乳铁蛋白的分离纯化 被引量：5

Isolation and Purification of Lactoferrin in Bovine Milk
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摘要 采用强阳离子交换色谱梯度洗脱法,对牛乳中的乳铁蛋白进行分离和纯化,并利用SDS-PAGE定性检测。结果表明:采用强阳离子交换色谱梯度洗脱法可较好地分离提取出乳铁蛋白,分离出的乳铁蛋白显示为单一区带,每毫升牛乳能提取乳铁蛋白0.23 mg,纯度为95%。 A gradient elution procedure for isolation and purification of lactoferrin by strong cation exchange chromatography in this paper,and lactoferrin was analyzed qualitatively by SDS-PAGE.The result shows that lactoferrin could be isolated and purificated by strong cation exchange chromatography in this paper,the isolated lactoferrin as a single band in SDS-PAGE,lactoferrin could be obtained 0.23 mg from per milliliter bovine milk and the purity was 95 %.
作者 肖岚 张振宇 王熙 辛松林
机构地区 四川烹饪高等专科学校
出处 《食品研究与开发》 CAS 北大核心 2010年第11期193-196,共4页 Food Research and Development
基金 四川省教育厅青年基金项目(09ZB057) 成都大学肉品重点实验室一般项目(10R06)
关键词 乳铁 分离 纯化 牛乳 l：actoferrin isolation purification bovine milk
分类号 TS252.1 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献10

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共引文献71

同被引文献55

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引证文献5

二级引证文献20

食品研究与开发
2010年 第11期

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