摘要
采用强阳离子交换色谱梯度洗脱法,对牛乳中的乳铁蛋白进行分离和纯化,并利用SDS-PAGE定性检测。结果表明:采用强阳离子交换色谱梯度洗脱法可较好地分离提取出乳铁蛋白,分离出的乳铁蛋白显示为单一区带,每毫升牛乳能提取乳铁蛋白0.23 mg,纯度为95%。
A gradient elution procedure for isolation and purification of lactoferrin by strong cation exchange chromatography in this paper,and lactoferrin was analyzed qualitatively by SDS-PAGE.The result shows that lactoferrin could be isolated and purificated by strong cation exchange chromatography in this paper,the isolated lactoferrin as a single band in SDS-PAGE,lactoferrin could be obtained 0.23 mg from per milliliter bovine milk and the purity was 95 %.
出处
《食品研究与开发》
CAS
北大核心
2010年第11期193-196,共4页
Food Research and Development
基金
四川省教育厅青年基金项目(09ZB057)
成都大学肉品重点实验室一般项目(10R06)
关键词
乳铁
分离
纯化
牛乳
l:actoferrin
isolation
purification
bovine milk