几株具有农药活性的海洋微生物菌株诱导番茄抗盐作用与机理研究

Marine Microorganisms Producing Pesticide Induce Salt Tolerance in Tomato and Its Mechanism

针对目前国家对盐渍地开发利用的重大技术需求,选用8株具有农药潜力的海洋微生物菌株,以番茄为主要目标植物,在盐害胁迫下对海洋微生物诱导番茄植株抗盐活性及植物体内外的Na+、K+浓度平衡进行了研究。结果表明,4株海洋微生物菌株具有较明显的诱导植物抗盐和缓解盐害的作用,但表现各有差异,其中FC02菌株对发芽率、发芽指数诱导作用最为明显,发芽指数提高了38.3%,幼苗的株高、叶长、展开叶数、茎粗分别提高82.2%、66.7%、30.76%和50%,CAT酶活性最高为3.03U.mg-1.min-1FW,比对照提高了144.1%;YM菌株促使番茄幼苗叶绿素总含量增加的作用最大,比对照提高了88.9%;B9987菌株在使番茄幼苗丙二醛含量降低48.6%的同时,显著改善了植物体内的K+、Na+浓度平衡,K+含量比对照提高了36.09%,Na+比对照降低了39.5%。上述研究结果显示,一些海洋微生物菌株具有良好的诱导植物抗盐作用。

The exploiture and utilization of saline soil is an urgent technical demand in China.In this study,8 marine microorganism strains with pesticides potential were selected to investigate their inducing ability of salt tolerance in tomato under salt stress.Physiology and biochemistry changes might occur when plants under salt stress.In this study,the induced salt tolerance of tomato and Na＋,K＋ concentration balance in vitro and in vivo by 8 marine microorganism strains was investigated.The results indicated that 4 out of the 8 strains could increase salt tolerance and relieve salt damage in different aspects.Specifically,as to strain FC02,tomato seedling germination rate,germination index,plant height,leaves length,number of leaf expansion,stem diameter increased 38.3%,82.2%,66.7%,30.76% and 50% respectively and the CAT enzymatic activity reached to 3.03 U.mg-1.min-1 FW,increased 144.1% compared to control.Strain YM possessed the most obvious inducing ability in augmenting seedling chlorophyl content with increase of 88.9%.Strain B9987 not only lowered 48.6% malondialdehyde content in seedling,but also ameliorated Na＋,K＋ concentration balance in vivo with K＋ concentration increased 36.09% and Na＋ concentration decreased 39.5%.These results directly and indirectly confirmed the preferable capacity of marine microorganisms to induce plant salt tolerance.