黑麦草-耳葡萄球菌对水体中Cu的联合修复效应研究

The Combined Remediation Effects of Lolium perenne L.-Staphylococcus auricularis on Cu Contaminated Water

采用水培实验方法模拟不同程度的Cu污染状况,研究了黑麦草与耳葡萄球菌联合修复作用对Cu的去除效果。结果表明,联合修复作用对Cu的去除效果明显优于单一的黑麦草修复,Cu初始浓度在5-30mg.L-1时,联合修复对Cu的去除率均达85%以上。耳葡萄球菌对Cu表现出较强的耐受性和吸附性,Cu浓度为100mg.L-1时其干重积累量达到40.6mg.g-1。耳葡萄球菌的存在降低了黑麦草根系及茎叶中Cu的积累量,联合修复组的黑麦草的生物量和叶绿素含量明显高于单一黑麦草修复组。该菌在一定程度上减轻了黑麦草所受Cu的毒害性,提高了水中Cu的联合修复效果。

The removal of Cu by combined Lolium perenne L.and Staphylococcus auriculari was studied using hydroponic culture experiments to simulate different levels of Cu contamination.The experimental results indicated that S.auricularis played an important role in plant-microorganism combined bioremediation.The overall removal effect of Cu by jointed Lolium perenne L.and S.auricularis was over 85% when the initial Cu concentrations in the water body were between 5～30 mg.L-1,which was more efficient than the single remediation by Lolium perenne L..Staphylococcus auricularis＇s better resistence and adsorption toward Cu were revealed and up to 40.6 mg.g-1 of Cu（DM）was observed in the shoots of Lolium perenne L.when treated with 100 mg.L-1 Cu.The Cu-biosorption capacity of S.auricularis consequently resulted not only in the increase of chlorophyll content in the leaves and plant biomass but also in the decreases of Cu concentrations both in the roots and shoots of Lolium perenne L..The investigation demonstrated that S.auricularis could protect Lolium perenne L.against heavy metal toxicity and enhance the combined bioremediation efficiencies of metal in the contaminated water.