L-蛋氨酸多吡啶铜(Ⅱ)三元配合物的合成及与DNA的相互作用

Synthesis and DNA-binding of Copper(Ⅱ) Complexes of L-methionine with Polypyridyl

文章合成了三种L-蛋氨酸多吡啶铜(II)三元混配配合物,[Cu(Met)(Phen)(ClO4)](ClO4)(1)(Phen=1,10-菲咯啉),[Cu(Met)(IP)(ClO4)](ClO4)(2)(IP=咪唑并[4,5-f]1,10-菲咯啉)和[Cu(Met)(PIP)(ClO4)](ClO4)(3)(PIP=2-苯基-咪唑并[4,5-f]1,10-菲咯啉)。采用电子吸收光谱和荧光光谱等方法研究了配合物与小牛胸腺DNA的相互作用,用凝胶电泳方法研究了配合物对pBR322DNA的氧化切割活性。结果表明,虽配合物与DNA的作用较弱,但多吡啶配体的平面大小可直接影响到配合物与DNA的相互作用,而氨基酸辅助配体可起到进一步的调控作用。

Three ternary copper（II） complexes,[Cu（Met）（Phen）（ClO4）]（ClO4）（1）（Phen=1,10-phenanthroline）,[Cu（Met）（IP）（ClO4）] （ClO4）（2）（IP=imidazo [4,5-f][1,10]phenanthroline） and [Cu（Met）（PIP）（ClO4）]（ClO4）（3）（PIP= 2-phenylimidazo[4,5-f][1,10]phenanthroline were synthesized.The interactions of the complexes with calf thymus DNA were investigated by electronic absorption spectroscopy and ethidiumbromide fluorescence spectroscopy.The oxidative DNA cleavage activities were evaluated by agarose gel electrophoresis method.The results indicated that although the DNA binding affinity were weak,the size of polypyridyl plane can directly affect the interaction of the complexes with DNA and the amino acid ligand can regulate it.