摘要
目的:研究抑癌基因P16与子宫内膜腺癌的关系。方法:用免疫组化ABC法检测123份石蜡包埋的子宫内膜腺癌标本(Ⅰ期80份、Ⅱ期12份、Ⅲ期29份、Ⅳ期2份)和40份增生期子宫内膜标本;用多聚酶链反应单股构象多态性分析(PCR-SSCPA)10份子宫内膜腺癌P16基因外显子1、2点突变。结果:P16蛋白阳性表达率:增生期子宫内膜为775%,且均为高度阳性表达,子宫内膜腺癌为4308%,其中Ⅰ期为5125%、Ⅱ期为25%、Ⅲ期为31%、Ⅳ期为0;子宫内膜腺癌P16阳性表达率明显低于正常增生期子宫内膜者(P<005),且临床期别越晚,P16蛋白阳性表达率越低(P<005),阳性表达强度越弱(P<005),Ⅰ期明显高于Ⅱ、Ⅲ、Ⅳ期(P<005)。10份子宫内膜腺癌P16基因外显子1、2均未检测出点突变。结论P16基因蛋白的改变与子宫内膜腺癌的发生有关;临床期别越晚,其改变越明显,P16基因的改变可能不以点突变为主。
Objective: TostudyontherelationshipbetweensuppressorgeneP16andendometrialadenocarcinomaMethods:P16 proteinexpressionwasexaminedusing immunohistochemistryABCin123specimensofendomtrialadenocarcinoma(stage80specimens,stage12specimens,stage29specimens,andstage2specimens)and40specimensofnormalwomenendometrinm10specimensofendometrialadenocarcinomaweremeasuredforP16genepointmutationatexon1,2usingpolymerasechainreactionsinglestrandconformationpolymorphismanalysis(PCRSSCPA)Results:P16proteinpositiveexpressionratewas4308%inendometrialadenocorrcinoma(stage5125%,stage 25%,stage31%,stage0)andlowersignificantlythanthatinthenormalwomenendometriumTherateofP16proteinpositiveexpressionofstagewashighersignificantlythanthatinsatges,and (P<005) 10specimensofendometrialadenocarcinomawasnotfoundpointmutationConclusions:ThelossorabnormalityofP16proteinexpressionisrelatedwithendometrialadenocarcinomaanditsclinicalstageP16genealterationsinendometrialadencocarcimomamaynotmainlyoccurinmutation
出处
《现代妇产科进展》
CSCD
1999年第2期105-108,共4页
Progress in Obstetrics and Gynecology
关键词
P16
子宫内膜肿瘤
腺癌
抑癌基因
SuppressorgeneP16
Endometrilneoplasms,Adenocarcinoma
Immunohistochemistry