抑癌基因P16与子宫内膜腺癌关系的研究

AStudyontherelationshipbetweensuppressorgeneP16andendometrialadenocarcinoma

目的：研究抑癌基因Ｐ１６与子宫内膜腺癌的关系。方法：用免疫组化ＡＢＣ法检测１２３份石蜡包埋的子宫内膜腺癌标本（Ⅰ期８０份、Ⅱ期１２份、Ⅲ期２９份、Ⅳ期２份）和４０份增生期子宫内膜标本；用多聚酶链反应单股构象多态性分析（ＰＣＲ－ＳＳＣＰＡ）１０份子宫内膜腺癌Ｐ１６基因外显子１、２点突变。结果：Ｐ１６蛋白阳性表达率：增生期子宫内膜为７７５％，且均为高度阳性表达，子宫内膜腺癌为４３０８％，其中Ⅰ期为５１２５％、Ⅱ期为２５％、Ⅲ期为３１％、Ⅳ期为０；子宫内膜腺癌Ｐ１６阳性表达率明显低于正常增生期子宫内膜者（Ｐ＜００５），且临床期别越晚，Ｐ１６蛋白阳性表达率越低（Ｐ＜００５），阳性表达强度越弱（Ｐ＜００５），Ⅰ期明显高于Ⅱ、Ⅲ、Ⅳ期（Ｐ＜００５）。１０份子宫内膜腺癌Ｐ１６基因外显子１、２均未检测出点突变。结论Ｐ１６基因蛋白的改变与子宫内膜腺癌的发生有关；临床期别越晚，其改变越明显，Ｐ１６基因的改变可能不以点突变为主。

Objective: TostudyontherelationshipbetweensuppressorgeneP16andendometrialadenocarcinomaMethods:P16 proteinexpressionwasexaminedusing immunohistochemistryABCin123specimensofendomtrialadenocarcinoma(stage80specimens,stage12specimens,stage29specimens,andstage2specimens)and40specimensofnormalwomenendometrinm10specimensofendometrialadenocarcinomaweremeasuredforP16genepointmutationatexon1,2usingpolymerasechainreactionsinglestrandconformationpolymorphismanalysis(PCRSSCPA)Results:P16proteinpositiveexpressionratewas4308%inendometrialadenocorrcinoma(stage5125%,stage 25%,stage31%,stage0)andlowersignificantlythanthatinthenormalwomenendometriumTherateofP16proteinpositiveexpressionofstagewashighersignificantlythanthatinsatges,and (P<005) 10specimensofendometrialadenocarcinomawasnotfoundpointmutationConclusions:ThelossorabnormalityofP16proteinexpressionisrelatedwithendometrialadenocarcinomaanditsclinicalstageP16genealterationsinendometrialadencocarcimomamaynotmainlyoccurinmutation