不明原因早期自然流产患者蜕膜淋巴细胞的超微结构变化

Ultrastructurechangesofdeciduallymphocytesfrompatientswithunexplainedspontaneousabortion in the early trimester .

目的：探讨不明原因早期自然流产患者蜕膜中淋巴细胞的种类及其超微结构的变化。方法：以１０例正常妊娠早期施行人工流产的健康孕妇作为对照组，用光镜及扫描电镜对早期自然流产的１０份蜕膜组织中不同淋巴细胞的百分率及其超微结构进行检测。结果：实验组蜕膜淋巴细胞百分率平均为６３３５％±２１９％，其中，大颗粒淋巴细胞及小淋巴细胞在蜕膜细胞中所占的百分率分别平均为６００５％±１９２％、３３０％±０５８％；对照组相应百分率则分别平均为５６１０％±３４７％、５２３５％±３４２％、３７５％±０５９％，两组比较，实验组总淋巴细胞及大颗粒淋巴细胞百分率显著上升（Ｐ＜００００５），但小淋巴细胞百分率未见显著改变（Ｐ＞００５）。透射电镜观察结果表明，不论大颗粒淋巴细胞还是小淋巴细胞，实验组均表现出细胞功能活跃的超微结构变化，如胞质中细胞器丰富，含大量的溶酶体及大、小泡；细胞核形态各异，核孔较多，常见核仁，常染色质丰富等，而且实验组呈细胞凋亡形态学变化的淋巴细胞增多，与对照组相比分别平均为６４０％±１１７％，３１５％±０７５％（Ｐ＜００００５）。结论：不明原因早期自然流产患者蜕膜淋巴细胞的超微结构发生了?

Objective:Toobservethe ultrastructurechangesofdeciduallymphocytesfrompatientswithunexplainedspontaneous abortion in the early trimester.Methods: 10 specimens from thehealthypregnantwomenascontrols were compared with 10 samples of decidual tissues usingmicroscopeandtransmissionelectronmicroscope.Results:The averagepercentageoftotallymphocytein study roup washigherthanthatofcontrolgroup(63.35%2.19%v s56.10%3.47%, P<00005)TBZ,inwhichtheproportionsoflargegranularlymphocytes(LGL)were upregulatedsignificantely(6005%192% vs5235%342%, P<00005) ,andthatofsmalllymphocytehadnochange(330%058% vs3750.59%, P>005) .EitherLGLorsmalllymphocytehadultrastructurechangesofactivecellfunction,plentyoforganellae,lysosomes,largeandsmallvesicles;variousnucleuswithmorenuclearporesandnucleolus;greateramountofeuchromatin,etc.Moreover,thepercentageofapoptoticlymphocyteincreasedgreately(6.401.17%vs3.15%0.75%, P<00005)ZConclusion:Thesedatasupplied someexperimentalevidence ofcellularmorphologyforimmunopathogenesis ofspontaneousearlyabortion