老年人血管紧张素转化酶基因多态性与其血清水平及高血压病的关系

Association of Polymorphism of ACE in the Elderly and Its Serum Level with Hypertension

为探讨武汉地区汉族老年人血管紧张素转化酶（ACE）基因插入／缺失（I／D）多态性与血清ACE水平及高血压病的关系，随机选择41例正常老年人，35例老年高血压病患者，应用聚合酶链反应（PCR）技术检测其ACE基因型。同时，用分光光度法测定其中29例老年高血压病患者的血清ACE浓度。结果显示ACE基因I／D多态性与老年人高血压病无相关关系。但血清ACE水平在ACE三种基因型之间有显著差异，DD型者最高［（37．50±1．51）U／ml），显著高于ID型［（23．11±3．03）／U／ml」和Ⅱ型者［（20．59±2．87）U／ml］。提示在武汉地区汉族老年人群体中，ACE基因I／D多态性不是高血压病发病的遗传标志。但ACE基因缺失型高血压患者的血清ACE水平增高，这可能与该多态性参与介导其他心血管病的发生有关。

To identify the relationship between the insertion/deletion (I/D) polymorphism of angiotensin- converting enzyme (ACE ) gene and serum ACE levels, also to find whether the ACE gene polporphism is associated with hypertension in the elderly population of Chinese Han-nationality. ACE genotyping with opwnerase chain reaction (PCR) was performed randomly in 41 unrelated healthy controls and 35 hypertensive elder patients. Serum ACE levels were concomitantly measured by spectrophtometric assay in 29 hypertensives without ACE inhibitors in two monthes. The results showed that ACE gene I/D polymporphism is not associated with hypertension. However the serum ACE levels of DD genotype (37. 50± 1 . 51 U/ml) were the highest among the three genotypes (23. 11± 3. 03 U/ml, 20. 59 ±2. 87 U/ml for ID and Ⅱ genotype respectively). Conclusion: In Chinese Han-nationality elderly population, ACE gene I/D polymorphism is not the hereditary factor of hypertension, but serum ACE levels were controled by ACE genotypes. The relationship between DD genotype and some cardiovascular diseases may be due to the higher serum ACE levels of deletion poymorphism.