OLP患者唾液和血清中骨桥蛋白等细胞因子的表达

Expression of osteopontin and other cytokines in saliva and serum in patients with oral lichen planus

目的:研究口腔扁平苔藓(OLP)患者唾液和血清中骨桥蛋白(OPN)等细胞因子的含量及其相互关系。方法:经病理学检查确诊的OLP患者26例,其中充血糜烂型12型,网纹/斑块型14例。酶联免疫吸附试验(ELISA)检测患者唾液和血清中OPN,肿瘤坏死因子-α(TNF-α),转化生长因子-β1(TGF-β1)的含量。以26名健康志愿者作为正常对照。结果:OLP患者唾液和血清OPN,TNF-α含量与正常对照组相比均有升高(P<0.05);充血糜烂型OLP的TGF-β1唾液和血清水平显著高于网纹/斑块型OLP(P<0.05)。三种抗体在唾液和血清中表现出明显的相关关系。结论:OPN在OLP患者唾液和血清中含量升高,唾液和血清中OPN含量具有相关性。唾液作为无创伤性检测标本有望有限地代替血清标本应用于OLP与口腔白斑的临床检测。

Objective：To explore the relationship of saliva and serum concentration of osteopontin（OPN）and other proinflammatory cytokines in patients with oral lichen planus（OLP）.Method：twenty-six patients with pathologically-confirmed OLP（erosive OLP,n=12;reticular OLP,n=14）were investigated.Saliva and serum levels of OPN,TNF-alpha and TGF-beta 1 were examined by enzyme linked immunosorbent assay（ELISA）.Twenty-six healthy subjects were served as normal controls.Result：Both saliva and serum OPN,TNF-alpha levels were increased in oral lichen planus patients in comparison with healthy subjects（P 0.05）.TGF-beta levels in erosive form were remarkedly increased than that in reticular form in both liquid（P 0.05）.Positive statistical correlations existed between these cytokines in saliva and serum.Conclusion：Both saliva and serum OPN level were increased in patients with OLP.there was correlation between saliva and serum OPN level,indicating that saliva perhaps displace partly serum determination as uninjured method be used in clincal diagonsis of OLP.