鱼腥草叶片总RNA提取方法研究

Extraction Methods of Total RNA from Houttuynia cordata Leaves

[目的]筛选出适合鱼腥草(Houttuynia cordata)叶片RNA提取的方法。[方法]以鱼腥草叶片为材料,比较了经典RNA提取试剂盒、Trizol试剂盒A、Trizol试剂盒B以及富含多糖类材料RNA提取试剂盒提取鱼腥草RNA的效果。[结果]Trizol试剂盒A、Trizol试剂盒B和经典RNA提取试剂盒难以提取出高质量的RNA,而富含多糖类材料RNA提取试剂盒提出的RNA质量高,完整性好,可以满足进一步分子生物学研究的要求。[结论]普通RNA提取试剂盒难以提取出鱼腥草叶片的RNA。

[Objective] The research aimed to screen suitable extraction method of total RNA from Houttuynia cordata.[Method] Four extraction methods,Classic Total RNA Isolation Kit,Trizol kit A,Trizol kit B and Easy Way RNA Amylose Plant MiNi Kit were compared for extracting total RNA from Houttuynia cordata.[Result] It was difficult to extract total RNA from Houttuynia cordata leaf by Trizol kit A,Trizol kit B and Classic Total RNA Isolation Kit method.Total RNA extracted by EasyWay RNA Amylose Plant MiNi Kit had high quality and could be use for the further research of molecular biology.[Conclusion] The ordinary RNA isolation Kit was hard to extract total RNA from Houttuynia cordata leaf.