河北省HIV-1流行株基因序列测定及亚型分析

Subtype and sequence analysis of the gag and env genes for HIV-1 strains isolated in Hebei Province

目的 了解河北省HIV-1流行株的亚型分布和流行趋势.方法 从感染者的血浆样品中提取病毒RNA,逆转录后采用套式PCR扩增HIV-1 gag和env基因的部分片段,对PCR产物直接进行核苷酸序列测定,所获序列与各亚型国际参考株序列比对,确定基因型并进行系统进化树分析.结果 对154份HIV-1感染者的样品进行扩增,得到了148份样品的HIV-1基因片段.发现6种HIV-1亚型和重组型,以及2例未定型.其中B＇亚型61例（41.2%）、CRF01_AE 59例（39.9%）、CRF07_BC 16例（10.8%）、CRF08_BC 6例（4.1%）、C亚和B01亚型各2例（1.4%）.在河北省首次发现了B01亚型.结论 2009年河北省存在多种HIV-1亚型和流行重组型,主要是B＇亚型和CRF01_AE重组型,应加强对HIV-1毒株亚型变异的监测,及时调整防治策略.

Objective To study the epidemic situation and characteristic of subtypes of HIV-1 strains prevalent in Hebei Province. Methods Viral RNA was extracted from plasma samples, HIV-1 genes （env and gag ） were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.The acquired sequences were compared with international subtypes references and their phylogenetic-trees were analyzed to determine the subtype. Results Among 154 HIV-1 antibody positive cases , 148 HIV-1 gene fragments were amplified and analyzed. There were 6 kinds of HIV-1 subtypes and recombinants, moreover unidentified 2 cases in 148 samples, of which 61 （41.2%） cases of B＇, 59（39.9% ） cases of CRF01_AE, 16（ 10.8% ） cases of CRF07_BC, 6（4.1% ） cases of CRF08_BC, and 2（ 1.4% ） cases of C and B01 each. HIV-1 B01 was detected firstly in Hebei Province. Conclusion Six HIV-1 subtypes were identified in Hebei Province. B＇ and CRF01_AE are the primary subtypes and recombinants of HIV-1 existed in Hebei Province. The surveillance of HIV-1 gene variation should be paid more attention to.