局灶性脑缺血再灌注损伤大鼠外周血内皮祖细胞数量的变化及其意义

Change and its significance of the number of peripheral blood endothelial progenitor cells in rats with focal cerebral ischemic reperfusion injury

目的探讨局灶性脑缺血再灌注损伤(CIR)大鼠外周血内皮祖细胞(EPCs)数量的变化及其意义。方法 50只健康雄性SD大鼠随机分为正常对照组(5只)、假手术组(5只)、CIR模型组(15只)、糖尿病模型组(5只)、糖尿病假手术组(5只)和糖尿病合并CIR组(15只)。由链脲佐菌素(STZ)诱导制作糖尿病大鼠模型;采用线栓法制作CIR大鼠模型。各模型大鼠采用Longa评分标准进行神经功能评分。CIR模型组与糖尿病合并CIR组各取5只大鼠采用2,3,5-三苯基氯化四氮唑(TTC)染色,计算其脑梗死体积。用流式细胞仪计数各组大鼠外周血EPCs的数量。结果糖尿病合并CIR组大鼠神经功能评分[(2.8±1.0)分]明显高于CIR模型组[(1.5±0.3)分],且脑梗死体积[(464.1±169.3)mm3]明显大于CIR模型组[(101.3±57.4)mm3](均P<0.05)。各组中,CIR模型组大鼠外周血EPCs数量最多,糖尿病合并CIR组最少,正常对照组与假手术组多于糖尿病模型组与糖尿病假手术组(均P<0.01)。结论 CIR大鼠外周血EPCs数量增加,有助于修复血管及保护受损脑组织;糖尿病大鼠外周血EPCs数量明显减少,且合并CIR后减少更甚。外周血EPCs数量的检测有助于CIR病情及预后的评估。

Objective To investigate the change and its significance of the number of peripheral blood endothelial progenitor cells （EPCs） in rats with focal cerebral ischemic reperfusion （CIR） injury. Methods Fifty male healthy SD rats were randomly divided into normal control group （n = 5） ,sham operation group （n = 5） ,CIR group （n = 15 ） ,diabetic group （n = 5） ,diabetic with sham operation group （n = 5） and diabetic with CIR group （n = 15） . The diabetic models were induced by streptozotocin （STZ） . The CIR models were operated by thread occlusion method. The score of neurological function in each model group was detected by Longa score standard. CIR group and diabetic with CIR group were separately taken 5 rats to be stained by 2,3,5-Triphenyltetrazolium chloride （TTC） ,and then their infarction volume were measured. The number of peripheral blood EPCs was detected by flow cytometry. Results The score of neurological function in diabetic with CIR group（2. 8 ± 1. 0） was significantly higher than in CIR group （1. 5 ± 0. 3） ,and the infarction volume in diabetic with CIR group [（464. 1 ± 169. 3） mm3]was significantly larger than in CIR group [（101. 3 ± 57. 4） mm3 ]（all P 0. 05） . The number of peripheral blood EPCs was the most in CIR group,and it was the least in diabetic with CIR group,and it was more in normal control group and sham operation group than in diabetic group and diabetic with sham operation group （all P 0. 01 ） . Conclusions The number of peripheral blood EPCs in CIR rats is increased,which can help to repair damaged vessels and protect brain tissue. The number of peripheral blood EPCs in diabetic rats is decreased,and even less when complicated with CIR. The number of peripheral blood EPCs may be helpful to reflect the condition and prognosis of CIR.