PAC-1,L-OHP单用及联合用药对人结肠癌细胞的抑制作用

The inhibition of PAC-1,L-OHP and PAC-1 plus L-OHP on the human colorectal cancer cell

目的观察体外单用及两者联合应用半胱天冬酶原活化复合物-1(PAC-1)和奥沙利铂(L-OHP)对人结肠腺癌细胞株HCT116、SW480、HT-29,SW116和SW620的生长抑制作用。方法 MTT法检测两药单用和合用前后对细胞增殖活性的影响。结果 PAC-1和L-OHP单用对5种人结肠癌细胞均有明显的诱导凋亡和增殖抑制作用,并呈时间和浓度依赖性;PAC-1对上述细胞的半数抑制浓度(IC50)之间无统计学差异(P>0.05),L-OHP对上述细胞的的IC50值有统计学意义(P<0.05)。PAC-1与L-OHP联用(同时给药)产生拮抗作用(CI(1)。结论 PAC-1与L-OHP单用均对人结肠腺癌细胞有抑制作用,但各细胞株对两药的敏感性不同,两者联合应用(同时给药)起拮抗作用。

Objective To investigate the inhibition of procaspase-activating compound-1（PAC-1）,Oxaliplatin（L-OHP） and PAC-1 conbination with L-OHP on human colorectal cancer cell lines （HT-29,SW116,HCT116,SW620,SW480）.Methods MTT assay was used to investigate the proliferation of cells before and after single and combined drug use.Results Both PAC-1 and L-OHP had signficant high effect of inducing apoptosis and inhibiting proliferation on five human colorectal cancer cells on a dose and time dependent manner.The IC50 of PAC-1 on different human colorectal cancer cells had no statistical significance（P0.05）; but IC50 of L-OHP on the 5 cell lines had statistical significance（P0.05）.PAC-1 combined with L-OHP （dosing pump at the same time）had an antagonistic effect（CI﹥1）.Conclusions Both PAC-1 and L-OHP can inhibit human colorectal cancer cell lines,but the susceptibility of individual colorectal cancer cell lines on the two drugs are different.PAC-1 combined with L-OHP has an antagonistic effect.