结肠癌中TSPAN-1和CORTACTIN蛋白的表达及其临床意义

Expression and clinical significance of TSPAN-1 and CORTACTIN in colorectal carcinoma

目的研究TSPAN-1和CORTACTIN基因在结直肠癌组织中的表达及其与临床病理因素的关系。方法采用免疫组化Elivision二步法,检测TSPAN-1和CORTACTIN基因蛋白在80例结直肠癌组织,13例结肠腺瘤组织及27例癌旁正常结肠黏膜组织中的表达。结果 TSPAN-1蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为7.4%、23.0%、90.0%,组间差异具有统计学意义(P<0.01);CORTACTIN蛋白在正常黏膜、结肠腺瘤、结肠癌组织中阳性表达率分别为52.0%、77.0%、97.5%,组间差异均具有统计学意义(P<0.01);TSPAN-1和CORTACTIN的表达与结肠癌的淋巴结转移、TNM分期、5年生存率、组织学分级及浸润深度有关(P<0.01);TSPAN-1和CORTACTIN在结肠癌组织中的表达强度呈正相关(r=0.397,P=0.0013),二者表达的一致率高达77.8%(62/80)。结论 TSPAN-1和CORTACTIN基因的高表达与结肠癌的侵袭和发展有关,二者在结肠癌的细胞增殖、浸润和转移中有协同作用。联合检测二者的表达对于进一步理解结肠癌的生物学行为和判断预后有一定价值。

Objective To study the expression of TSPAN-1 and CORTACTIN protein in colorectal carcinoma,and investigate the relationship between the expression and clinicopathological parameters of colorectal carcinoma.Methods Immunohistochemical Elivision two-step method was used to detect the expression of TSPAN-1 and CORTACTIN protein in 80 cases of colorectal carcinoma,13 cases of colorectal adenoma and 27 cases of normal colorectal tissues.Results The positive expression rate of TSPAN-1 and CORTACTIN in colorectal carcinoma was significantly higher than that in colorectal adenoma and normal colorectal tissues,and there was significant difference in expression of every group （P0.01）.The positive expression rate of TSPAN-1 and CORTACTIN was 90% and 97.5% in group colorectal carcinoma,23% and 77% in group colorectal adenoma and 7.4% and 52% in group normal colorectal tissues （P0.01）.The expression of TSPAN-1 and CORTACTIN was positively related with lymph node metastasis,TNM stage,5-year survival rate,histopathological grading and depth of tumor invasion （P0.01）.The expression of TSPAN-1 was positively related with the expression of CORTACT in colorectal carcinoma （r=0.397,P=0.013）.Conclusions The over expression of TSPAN-1 and CORTACTIN gene are closely related to the invasion and growth of colorectal carcinoma,and TSPAN-1 and CORTACTIN have synergistic effect on infiltration,invasion and metastasis of malignant cells.Detecting the expression of both TSPAN-1 and CORTACTIN may be of value in further understanding the biological behavior and predicting the prognosis of colorectal carcinoma.