活血化瘀注射液等对急性胰腺炎大鼠血清一氧化氮浓度的影响

Effect of Huoxue Huayu Injection on Serum Nitric Oxide Concentration of Rats with Acute Pancreatitis

目的：于急性胰腺炎（ＡＰ）大鼠观察了活血化瘀注射液（ＨＨＩ－Ｉ）等对血清一氧化氮（ＮＯ）浓度的影响。方法：大鼠ＡＰ应用胆胰管内逆行注射牛磺酸钠溶液的方法引起。收集对照与治疗各组的血标本并应用ＮＯ测定试剂盒分别测定治疗前后的血清ＮＯ浓度。结果：丹参注射液（４例）未能降低ＡＰ所致的血清ＮＯ升高（Ｐ＜０．０５），赤芍注射液（４例）能维持血清ＮＯ于疾病前水平（Ｐ＞０．０５），而ＨＨＩ－Ｉ（６例）则使之明显降低（Ｐ＜０．０５），复方ＨＨＩ－Ｉ（ＨＨＩ－ＩＣＯ，６例）降低血清ＮＯ的作用更强（Ｐ＜０．０１）。结论：ＨＨＩ－Ｉ等对ＡＰ大鼠血清ＮＯ的升高有明显降低作用。

Objective:To observe the effect of Huoxue Huayu injection, etc.on serum concentration of nitric oxide in rats with acute pancreatitis.Methods:Rat pancreatitis was induced by retrograde infusion of taurocholate solution into biliopancreatic duct. Blood samples of the control and treatment groups were collected and nitric oxide concentrations before and after pancreatitis were determined. Results:Danshen in jection(4 cases) could not decrease the increased level of nitric oxide induced by pancrestitis ( P<0.05 ).ChiShao injection(4 cases)could maintain the nitric oxide concentration at the level before pancreatitis( P>0.05 ).Whereas, Huoxue Huayu injection(6 cases) could decrease the high level( P<0.05 )and the composite Huoxue Huayu injection(6 cases)had even more potent reducing effect on serum nitric oxide concentration( P<0.01 ).Conclusion:Huoxue Huayu injection and the like have significant reducing effect on serum nitric oxide conentration of rats with acute pancreatitis.