摘要
目的探讨抗Fas单克隆抗体诱导胃癌细胞凋亡的规律及在胃癌治疗中的意义.方法应用细胞形态观察、琼脂糖凝胶电泳、流式细胞光度术检测抗Fas单克隆抗体对胃癌细胞SGC7901增殖周期的影响以及对细胞杀伤作用的方式,并检测了SGC7901细胞表面Bcl2的表达情况..结果抗Fas单克隆抗体有阻滞细胞周期、通过诱发凋亡而抑制肿瘤细胞生长的作用.经抗Fas单克隆抗体处理后,SGC7901细胞表面Bcl2蛋白表达无明显变化..结论抗Fas单克隆抗体可以诱导胃癌细胞系SGC7901细胞凋亡.
AIM To investigate the pattern of anti Fas monoclonal antibody induced apoptosis and its role in treatment of gastric carcinoma. METHODS Anti Fas monoclonal antibody was tested for its effect on cell cycle and cell injury in gastric carcinoma cell line SGC 7901 with cell morphology, agarose gel electrophoresis and flow cytometry. Bcl 2 protein expression in SGC 7901 cells was also examined. RESULTS Anti Fas monoclonal antibody could block tumor cell proliferation cycle and trigger cell death by inducing apoptosis. The expression levels of Bcl 2 did not correlate with the apoptotic index after treatment with anti Fas antibody. CONCLUSION Anti Fas monoclonal antibody could induce human gastric carcinoma cell line SGC 7901 apoptosis. The biological effect did not correlate with endogenous Bcl 2 expression.
出处
《世界华人消化杂志》
CAS
1999年第6期476-478,共3页
World Chinese Journal of Digestology
基金
军队医药卫生九五重点课题资助项目
关键词
胃肿瘤
细胞凋亡
单克隆抗体
stomach neoplasms
apoptosis
antibodies, monoclonal