重组反义c-myc腺病毒对人胃癌细胞的体内及体外分子治疗

In vitro and in vivo molecular therapy with ASc myc adenovirus for human gastric carcinoma cell line *

目的研究重组反义ｃｍｙｃ腺病毒对胃癌细胞的体内外生物学作用．方法采用ＬａｃＺ基因Ｘｇａｌ染色、ＭＴＴ，ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪、ＰＣＲ分析、裸鼠致瘤性、裸鼠皮下移植瘤模型实验等方法，对反义ｃｍｙｃ重组腺病毒在人胃癌ＳＧＣ７９０１细胞系中的作用进行体内外研究．结果ＡｄＡＳｃｍｙｃ对ＳＧＣ７９０１细胞能产生明显的生长抑制作用，ＭＴＴ显示生长抑制率为４４１％．ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪显示ＡｄＡＳｃｍｙｃ诱导了ＳＧＣ７９０１细胞凋亡．经ＡｄＡＳｃｍｙｃ处理的ＳＧＣ７９０１细胞裸鼠致瘤性消失．ＡｄＡＳｃｍｙｃ对裸鼠皮下移植瘤模型瘤内注射能有效降低肿瘤的生长速度，生长抑制率为６８９％．

AIM To study the biological effects of recombinant antisense c myc adenovirus on human gastric cancer cell line in vitro and in vivo . METHODS Human gastric cell line SGC7901 was treated with Ad AS c myc or Ad LacZ X gal stain, MTT, DNA ladder, Tunel, FCM, PCR, the tumorigenicity in nude mice and experimental therapy on the nude mice model were studied. RESULTS Ad AS c myc could strongly inhibit cell growth and induce apoptosis of SGC7901 cells. The inhibitory rate of the Ad AS c myc infected SGC7901 cells was 44 1%. The tumorigenicity in nude mice using Ad AS c myc showed that all three mice failed to form tumors from 7 to 30 days as compared with Ad LacZ and parent SGC7901 cells. Experimental therapy on the nude mice model bearing subcutaneous tumor of SGC7901 cells showed that intratumor instillation of Ad AS c myc inhibited the growth of the tumors. Ad AS c myc treated tumors were inhibited by 68 9%,compared with the tumors injected with Ad LacZ and mock. CONCLUSION The expression of Ad AS c myc can inhibit growth and induce apoptosis of gastric cancer cells in vitro and in vivo.