幽门螺杆菌flaA基因的变异

Variability of fla A gene of Helicobacter pylori

目的鞭毛是幽门螺杆菌（Ｈｐ）的重要侵袭因子，本研究拟检测Ｈｐ鞭毛素Ａ基因的变异性，进而研究其与Ｈｐ致病性的关系．方法我们对５２例胃粘膜活检标本和１８株Ｈｐ临床分离株进行Ｈｐ特异的１６ＳｒＲＮＡ基因和鞭毛素Ａ基因ＰＣＲ扩增和ＰＣＲＲＦＬＰ及ＰＣＲＲＦＬＰＳＳＣＰ分析．结果对４３例Ｈｐ（＋）的ｆｌａＡ基因ＰＣＲＲＦＬＰ（ＨｉｎｄⅢ）大约可分７个型（变异检出率为１６３％），而ＰＣＲＲＦＬＰＳＳＣＰ可分３７个型（变异检出率为８６０％），两者有非常显著性差异．结论ｆｌａＡ基因变异性极大，ＰＣＲＲＦＬＰＳＳＣＰ优于ＰＣＲＲＦＬＰ，是检测其变异的有效方法．

AIM To study the variability and pathogenjcity of Helicobacter pylori (Hp) . METHODS PCR was performed in fifty two biopsy specimens of gastric mucosa and 18 strains of Hp for 16S rRNA gene and flagellin A gene ( fla A). And PCR-RFLP and RFLP SSCP were also analyzed. RESULTS Seven fla A genotypes were found by PCR RFLP in 43 strains of Hp (detecting rate of variance was 16 3%), but 37 were found by PCR RFLP SSCP in the same group of cases (detecting rate of variance was 86 0%). CONCLUSION Very large variability in fla A gene of Hp was detected by PCR RFLP SSCP, which is a more effective method than PCR RFLP to identify Hp strains in human stomach.