化疗及导向化疗治疗大肠癌与癌细胞凋亡研究

Effect of chemotherapy or targeting chemotherapy on apoptosis of colorectal carcinoma

目的研究化疗及导向化疗对大肠癌组织及细胞的影响．方法大肠腺癌患者３７例，根据是否注射化疗药物分成Ａ，Ｂ两组．Ａ组１７例，术前不注射任何化疗药物或化疗药物的偶联物，将其作为对照组；Ｂ组２０例，其中７例手术前３ｄｉｖ丝裂霉素Ｃ（ＭＭＣ８ｍｇ），１３例手术前ｉｖ导向化疗药物抗ＣＥＡ单克隆抗体－ＭＭＣ交联物（含ＭＭＣ８ｍｇ，抗ＣＥＡ单克隆抗体１０ｍｇ）；其后对所有３７例患者行手术治疗并切取标本进行病理组织学观察，ＡｇＮＯＲ颗粒检测及肿瘤浸润淋巴细胞分类及计数．结果Ａ组病理退变程度１７例（１０００％）均为１级；Ｂ组病理退变程度１级４例（２００％），２级１２例（６００％），３级４例（２００％），两组之间差异有高度显著性（Ｐ＝０００００００３７６）．Ａ组癌细胞ＡｇＮＯＲ颗粒／核均数为４１１颗；Ｂ组癌细胞ＡｇＮＯＲ颗粒／核均数为２５８颗，其差异有高度显著性（Ｐ＜００１，ｔ＝５１４３）．肿瘤浸润淋巴细胞以Ｔ细胞ＣＤ＋３为主，同组中Ｔ淋巴细胞ＣＤ＋３（ａＰ＜００１，ｔ＝２０２２９）与Ｂ淋巴细胞ＣＤ＋２０之间的差异有高度显著性（ｂＰ＜００１，ｔ＝１９７６１）；Ａ组Ｔ淋巴细胞ＣＤ＋３为６０９１％，Ｂ组Ｔ?

AIM To study the effect of chemotherapy and targeting chemotherapy on colorectal carcinoma (CRC). METHODS Thirty seven patients were divided into two groups: group A (17 patients, without preoperative chemotherapy) and group B (20 patients, receiving preoperative chemotherapy, targeting chemotherapy and mitomycin C 8 mg ). Resected specimens of colorectal carcinoma were assessed histologically by silver staining protein score of the nucleolar organizer region (AgNOR) and by classification and counting of infiltrating lymphocytes to study apoptosis of neoplastic cells caused by chemotherapy and targeting chemotherapy. RESULTS In group A, all resected tumor tissues of 17 patients were in grade 1 retrograde degeneration, but in group B, 4 cases in grade 1, 12 cases in grade 2 and 4 cases in grade 3. There was a significant difference between the two groups ( P =0 000000376). Counts of AgNOR granules per nucleus in group A were 4 11 and 2 58 in group B with a significant difference ( t =5 143, P <0 01). The infiltrating lymphocytes were predominantly T lymphocytes (CD + 3), with a significant difference in a same group between counts of T lymphocytes ( t =20 229, a P <0 01) and B lymphocytes (CD + 20 ) ( t =19 761, b P <0 01). T lymphocytes was 60 91% in group A and 72 34% in group B. B lymphocytes was 10 78% in group A and 12 65% in group B. CONCLUSION Chemotherapy and targeting chemotherapy could cause retrograde degeneration of tumor tissues, decrease counts of AgNOR granules per nucleus of tumor cell, and cause the apoptosis of tumor cells. Infiltrating lymphocytes were predominantly T lymphocytes (CD + 3) after chemotherapy and targeting chemotherapy.