晚期糖基化终末产物及其受体在瘢痕疙瘩中的表达

Expressions of advanced glycation end products and their receptors in keloid

目的 研究晚期糖基化终末产物（AGE）及其受体（AGER）在瘢痕疙瘩中的表达.方法 瘢痕疙瘩、增生性瘢痕和正常人群血清、皮肤组织标本各20份,以荧光分光光度计检测三组人群血清中AGE含量,分别采用免疫组化法、Western印迹分析检测三组人群皮肤组织标本中AGE和AGER表达情况.结果 瘢痕疙瘩组血清中AGE含量为（0.713±0.098）AU/ml,增生性瘢痕组为（0.699±0.077）AU/ml,明显高于正常人群组（0.179±0.056）AU/ml,三组差异有统计学意义（F=283.82,P〈0.01）.免疫组化显示,AGE、AGER在瘢痕疙瘩、增生性瘢痕皮肤组织标本中表达阳性,正常人群组织表达则为阴性.Western印迹检测显示,瘢痕疙瘩、增生性瘢痕组织中AGE和AGER蛋白表达均明显高于正常人群,差异有统计学意义（F值分别为18.04、42.80,P〈0.05）,而瘢痕疙瘩和增生性瘢痕组之间差异无统计学意义（P〉0.05）.结论 AGE和AGER在瘢痕疙瘩中表达升高,在其发病过程中可能发挥一定的促进作用.

Objective To investigate the expressions of advanced glycation end products （AGEs） and their receptors in keloid. Methods Serum and skin tissue specimens were collected from 20 patients with keloid, 20 patients with hyperplastic scar and 20 normal human controls. Fluorospectrophotometer was used to measure the serum level of AGEs, and immunohistochemistry and Western blotting to detect the expressions of AGEs and AGER in skin tissue specimens. Results The serum level of AGEs was （0.713 ± 0.098） AU/ml and （0.699 ± 0.077） AU/ml respectively in patients with keloid and those with hypertrophic scar, significantly higher than that in normal controls （0.179 ± 0.056 AU/ml, F = 283.82, P 〈 0.01 ）. A positive expression of AGEs and AGER was observed in tissue specimens of keloid and hyperplastic scar, but not in the control specimens. As Western blotting showed, the expressions of AGEs and AGER were higher in tissue specimens of keloid and hyperplastic scar than in the control specimens （F = 18.04, 42.80, both P 〈 0.05）, while no significant difference between keloid and hyperplastic scar tissue specimens was observed （P〉 0.05）. Conclusion There is a high expression of AGEs and AGER in keloid, which may contribute to the development of keloid.