胃癌p53和nm23基因异常表达的预后意义研究

Prognostic Significance of Abnormal Expression of p53 and nm23 in Gastric Cancer

目的研究胃癌ｐ５３和ｎｍ２３基因异常表达与胃癌生物学行为及病人预后的关系。方法采用链霉素—生物素（ＳＰ）免疫生化技术行ｐ５３和ｎｍ２３免疫组化染色，研究了２０９例胃癌病人的临床病理资料。结果ｐ５３和ｎｍ２３基因的异常表达率分别为４９．７６％和５７．４２％，ｐ５３及ｎｍ２３异常表达与病人性别、年龄、肿瘤部位、大小无关。随着ＴＮＭ分期的进展ｐ５３基因异常表达率升高（Ｐ＝０．００９），Ｂｏｒｍａｎｎ４胃癌ｎｍ２３异常表达率高（Ｐ＝０．０２６），ｐ５３在粘液腺癌低表达（Ｐ＝０．０１８６），ｐ５３及ｎｍ２３与胃癌的淋巴结转移有关（Ｐ＝０．０１８，Ｐ＝０．００２）。ｐ５３及ｎｍ２３与术后生存时间有关。结论ｐ５３及ｎｍ２３基因异常表达能反映胃癌的生物学行为，并与胃癌的预后有关。ｎｍ２３更能提示胃癌转移，但ｐ５３提示预后的能力比ｎｍ２３强。

Objective To study the prognostic significance of abnormal expression of p53 and nm23 in gastric cancer.Methods With LSAB immunohistochemical methods,209 cases of gastric cancer was studied.Results The abnormal expression rate of p53 and nm23 is 49.76% and 57.42% ,respectively.The presence of p53 and nm23 protein was not correlated with the sex and age of the patients,the location and size of the tumor,but they correlated with tumor lymphnode metastasis,and patients′ prognosis.Conclusions The abnormal expression of p53 was a better indicator in predicting prognosis.Determination of p53 and nm23 proteins immunohistochemically might be useful in predicting biologic behavior and prognosis of gastric cancer.The abnormal expression of nm23 was much more correlated with metastatic tendency.