N-乙酰神经氨酸和N-葡萄糖代神经氨酸对兔红细胞膜钙调蛋白活性的影响

The influence of Nacetylneuraminic acid and Nglycolylneuraminic acid for calmodulin on rabbit red cells

目的：比较唾液酸单体间的生物活性和揭示唾液酸与吗丙嗪间的关系。方法：以兔红细胞膜上钙调蛋白作为手段。结果：Ｎ乙酰神经氨酸（５ＡｃＮｅｕ，＞２００μｍｏｌ／Ｌ）和Ｎ葡萄糖代神经氨酸（ＮｅｕＧｃ，＞５０μｍｏｌ／Ｌ）能在体外显著抑制兔红细胞膜钙调蛋白（ＣａＭ）活性（Ｐ＜０．０５）。抑制率与所用浓度基本呈对数线性。ＮｅｕＧｃ的抑制强度约为５ＡｃＮｅｕ的２～４倍。此外５ＡｃＮｅｕ（２００μｍｏｌ／Ｌ）能使低浓度的吗丙嗪（２５～１００μｍｏｌ／Ｌ）增加对ＣａＭ的抑制率，因为在这一浓度范围内，吗丙嗪不能显示抗兔红细胞ＣａＭ的活性。结论：本工作进一步证明唾液酸单体具抗细胞钙调蛋白作用。吗丙嗪可能通过唾液酸产生药理活性。

Objective: To compare the biological activity of sialic acid in single forms and mechanism of the correlation between sialic acid and probimane. Methods: Calmodulin activity on rabbit red cell membrane were used. Results: Nacetylneuraminic acid (5 AcNeu,>200 mol/L)and Nglycolylneuraminic acid (NeuGc,>50 mol/L)could significantly inhibit the calmodulin activity on rabbit red cells in vitro(P<0.05).There is a logarithmic linear relation between neuraminic acid concentrations and inhibitory rates.The values of inhibitory rates of NeuGc was approximately 24 times higher than that of 5AcNeu.2 5AcNeu(200 mol/L)could enhance the inhibition of probimane (at low concentrations 25100 mol/L)for calmodulin.]Conclusion: Sialic acid in single form can inhibit calmodulin activity in certain concentration.There is relationship between sialic acid and probimane in calmodulin system.