用三种逆转录PCR扩增系统检测丙型肝炎病毒RNA的比较

Comparison of the detection with three RTPCR systems for HCV RNA

目的：比较常用的三种（ＭＭＬＶ／Ｔａｑ，ＡＭＶ／Ｔａｑ和Ｔｔｈ单酶）逆转录ＰＣＲ扩增系统的效果。方法：利用这三个系统对丙型肝炎病毒不同稀释度ＲＮＡ进行逆转录ＰＣＲ扩增，比较其检测灵敏度。结果：ＡＭＶ／Ｔａｑ系统最佳，ＭＭＬＶ／Ｔａｑ系统次之，Ｔｔｈ单酶系统最差，ＡＭＶ／Ｔａｑ系统的检测灵敏度比Ｔｒｈ单酶系统高１００００倍，比ＭＭＬＶ／Ｔａｑ系统高１００倍。结论：建议使用ＡＭＶ／Ｔａｑ酶的逆转录ＰＣＲ扩增系统以提高丙型肝炎病毒的检测灵敏度，减少假阴性的出现。

Objective: In order to compare three conventional reverse transcription polymerase chain reaction (RTPCR) systems (AMV/Taq,MMLV/Taq and Tth). Methods: A series of diluted HCV RNA was amplified by using three RTPCR systems. Results: AMV/Taq system is the most sensitive,Tth the most insensitive.The detecting sensibility of AMV/Taq system is 10 000 times more than that of Tth. Conclusion: Using AMV/Taq RTPCR for HCV RNA is suggested,to reduce false negative and increase detecting sensibility.