民间接骨中药方对大鼠骨折愈合的作用

Effects of prescribed Chinese folk herbal medicine on closed fracture rat

目的探讨民间中药方对大鼠闭合性骨折的治疗作用及作用机制。方法 48只SD大鼠胫骨骨折后随机分为3组:生理盐水组、伤科接骨片组、接骨中药组,每组16只。通过胫骨X线片,检测血清钙、磷、碱性磷酸酶等指标,并观察药物的疗效。结果接骨中药组血钙浓度随着实验时间的延长逐渐下降,在28d时明显下降,与术前相比,差异有统计学意义(P<0.05)。术后第28天接骨中药组碱性磷酸酶(ALP)活性明显低于生理盐水组,差异有统计学意义(P<0.05)。X线摄片显示3组间影像学差异无统计学意义(P>0.05)。结论接骨中药方可能有抑制骨吸收的作用,但对大鼠骨折的愈合作用有待进一步研究。

Objective To study the effect and mechanism of prescribed Chinese folk herbal medicine on closed fracture. Methods 48 SD rats with tibia fracture were divide into 3 groups：physiologic saline group,traumatology and orthopaedics remedy group,Chinese herbal medicine remedy group. The serum calcium,phosphorus,alkaline phosphatase and X-ray of tibia were observed. Results The blood calcium concentration in the Chinese herbal medicine remedy group decreased gradually as the duration of the experiment prolongs and drastically decreased on 28th day,compared preoperative,there had significant difference（ P 0. 05）. Preoperative 28th day,the phosphatase（ ALP） in Chinese herbal medicine remedy group was lower than that in physiologic saline group,the difference was statistically significant（ P 0. 05）. X-ray shows no significant differences between the three groups（ P 0. 05）. Conclusion The prescribed Chinese herbal medicine may have the effect on the inhibition of bone absorption. However,its effect on rat fracture healing remains to be further studied.