肝必康胶囊体外抗乙肝病毒的实验研究

Experimental Study on the Anti-HBV Activity in Vitro of Ganbikang Capsules

目的:观察肝必康胶囊对乙肝病毒的抑制作用。方法:采用HepG 2.2.15细胞的培养,MTT比色法检测药物对细胞的毒性,定量PCR检测HBV-DNA,ELISA法检测HBsAg、HBeAg的含量,研究肝必康胶囊对HepG 2.2.15细胞的毒性,HBsAg与HBeAg表达及HBV-DNA含量的影响。结果:肝必康半数细胞毒浓度(TC50)为4.677 mg/mL,最大无毒浓度(TC0)为0.118 mg/mL。各组在用药后72、144 h细胞上清中HBV DNA拷贝数降低,肝必康在0.1 mg/mL时抑制率最高。各组用药后细胞培养上清液中的HBsAg和HBeAg浓度显著降低。肝必康在0.1 mg/mL时抑制率最高。肝必康各组含药血清在用药后72、144 h细胞上清中HBsAg和HBeAg浓度显著降低,高剂量组抑制率最高。显示明显抗HBV的作用,TI均大于2。结论:肝必康胶囊体外培养细胞实验证实其有较强的抗乙肝病毒作用,且毒性较低。

Objective: To observe the inhibition effect of Ganbikang capsules on the anti-HBV activity in vitro.Methods: HepG 2.2.15 cells culture and MTT colorimetric method were used to test the drug toxicity on cells.PCR was used to test HBV DNA,ELISA to test content of HBsAg and HBeAg to study the toxicity of Ganbikang capsules on the cells,the expression of HBsAg and HBeAg and the effect of HBV-DNA.Results: TC50 of Ganbikang capsules was 4.677 mg/mL,with 0.118 mg/mL of 0% non toxicity(TC0).The copy number of HBV DNA of each group in the cells decreased after the treatment in 72 and 144 h whereas Ganbikang capsules showed the highest inhibition rate at 0.1 mg/mL.HBsAg and HBeAg concentration in the cell culture significantly lowered whereas Ganbikang capsules showed the highest inhibition rate at 0.1 mg/mL.HBsAg and HBeAg concentration in medicinal serum significantly lowered after the treatment in 72 and 144 h whereas the largest dose group showed the highest inhibition rate.Anti-HBV effect was obvious.TI was averagely larger than 2.Conclusion: The experiment of Ganbikang capsules on the anti-HBV activity in vitro shows strong anti-HBV effect and low toxicity.