摘要
2009年全球暴发猪源甲型H1N1流感病毒疫情，上海地区于5月份诊断第一例输入性病例。本研究从上海地区较早发现的两个输入性甲型H1N1流感病例样本中分离出2株病毒，分别命名为A／Shanghai／37T／2009和A／Shanghai／71T／2009。MDCK细胞培养上清中的病毒通过电镜显示，该病毒的直径大约为60～80nm，具包膜，包膜表面有突起，呈现出正粘病毒颗粒形态特征。免疫荧光检测显示，MDCK细胞内病毒成分能与病人恢复期血清反应。2株病毒的全基因核酸序列和氨基酸序列与美国参考株California04具有高度的同源性；基于全基因的系统发育分析，确认此2株病毒属于2009年在全球暴发流行的新型H1N1甲型流感病毒。在此基础上的进一步研究发现，2株病毒可诱导A549细胞中抗病毒效应基因的表达；经Ⅰ型干扰素预处理的细胞可有效抵抗病毒的感染，提示其潜在的保护作用。综上所述，上海分离的2株病毒和2009年北美暴发的猪源甲型H1N1流感病毒是同源的，并且在病毒的致病性，药物的抗性和抗原性方面没有发生变异。
A new strain of Influenza A/ H1N1 viruses first identified in April 2009 has caused global pandemics. Shanghai identified its first case in May. The virus strain A/Shanghai/37T/2009 and A/Shanghai/ 71T/2009 were isolated from two imported cases. Electron microscopy view of virus particles from MDCK culture showed that it contained numerous enveloped and highly pleomorphic orthomyxovirus particles with a diameter range from approximately 60 to 80 nm and surrounded by a fi-inge of well-defined surface projection. Compared the nucleotide sequences and amino acid sequences of the two viral strains with those of the reference strain California04, highly homologous were observed between them. Phylogenetic analysis showed that two strains were highly genetically similar to those previously reported 2009 influenza A （H1N1） strains, suggesting the same origins for the two Shanghai strains and the 2009 S-OIVs. Additionally, stimulating A549 cells （carcinoma human alveolar basal epithelial cells） with the virus could induce the expression of antiviral genes. Pretreatment of IFN-α caused A549 to resist viral infection, suggesting the potential protective effect of IFN-α In a word, two Shanghai A（H 1N 1） influenza virus and North American 2009 Swine-Origin A （H 1N 1 ） influenza viruses were highly homologous, and there was no different in pathogenicity, drug resistance and antigenicity among them.
出处
《公共卫生与临床医学》
2010年第4期261-268,共8页
Public health and dinical medicine
