动物转基因研究中的技术方法

The Development of Transgenic Technology in Animals

转基因动物研究的深入为生物学的发展起到了巨大的推动作用。转基因新技术的不断出现,推动了转基因动物的发展。介绍了几种哺乳动物转基因研究中常见的方法,包括原核显微注射法、逆转录病毒感染法、胚胎干细胞介导法、生殖细胞介导法、基因打靶以及新的比较有应用前景的方法,如PiggyBac、iPS,锌指核酸酶法,并且分别对每种方法的优缺点进行了总结归纳。

Transgenic animal research plays an important role in the development of biology,and emergence of new technologies promote the development of transgenic animals.This article reviewed several approaches used in transgenic animal research,such as microinjection,retrovirus infection,embryonic,germinal cell,gene targeting,and some promising new methods,such as Piggy Bac,iPS,ZFN.Several advantages and disadvantages of each method were also summarized.