摘要
报道了一种简便的制备分子量大小为100-1000bp DNA marker的方法,其原理是以一段特异的DNA片段为模板,设计PCR引物,采用多重PCR的方法一次扩增100-1000bp系列条带,酚/氯仿抽提,乙醇沉淀,即可得到条带清晰的DNA marker。
A simple method that prepare DNA marker is reported in this paper.A specific DNA fragment was used as PCR template,and multiple PCR was used to amplify 100-1 000 bp series DNA fragments,and then were purified and electrophoresised in 2% agarose gels,the bands of the prepared DNA marker were clear and could be used in the molecular experiments.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第12期189-190,200,共3页
Biotechnology Bulletin
基金
河南省科技厅科技攻关资助项目(0624410041)
河南省教育厅自然科学基金(2008A310008)
