四株抗荔枝病原菌的芽孢杆菌的分离鉴定

Isolation and Identification of Four Bacillus Strains with Antagonistic Ability Against Litchi Pathogenic Fungi

荔枝果树根际土壤中筛选出4株芽孢杆菌OR-1、OR-2、OR-3、ON-6,都显示出抗荔枝病原菌的活性。采取对该菌株形态特征、培养特征、生理生化特征和遗传特性进行研究的方法,结果表明菌株与枯草芽孢杆菌(Bacillus subtilis)的特征一致;将4菌株的16S rDNA序列在GenBank中进行序列比对,结果亦显示其与Bacillus subtilis的16S rDNA的序列片段的相似性均达99%以上;以相似性为基础构建系统进化树,分析表明菌株与Bacillus subtilis同源关系最近。最终得出结论为菌株OR-1、OR-2、OR-3、ON-6为枯草芽孢杆菌。

Four strains of Bacillus OR-1,OR-2,OR-3,ON-6,were isolated from rhizosphere soil of litchi,all of which showed the inhibitory activity of against litchi pathogens fungi.The investigation of their formal,cultural and physiological characteristics indicated that they had the same characteristic with Bacillus subtilis.Comparis on of their 16S rDNA sequence with the data in GenBank,showed the similarity of 99% or more between the four strains and Bacillus subtilis.Phylogenetic tree based on the similarity was established and confirmed that the four strain had the closest relationship with Bacillus subtilis.Finally,the strain OR-1,OR-2,OR-3,ON-6 were identified to be Bacillus subtilis.