宫颈癌中p16^(INK4A)蛋白表达的意义

Significance of p16^(INK4A) expression in cervical carcinoma

目的探讨p16INK4A蛋白与宫颈癌发生发展以及临床病理指标的关系。方法采用免疫组化法检测宫颈鳞癌组织40例,宫颈上皮内瘤变(CIN)30例,宫颈癌旁正常宫颈组织25例中的p16INK4A表达。结果 p16INK4A在宫颈上皮内瘤变中阳性率为86.6%,宫颈鳞癌组织中阳性率均呈100%表达,正常宫颈组织中为阴性。结论 p16基因在宫颈癌组织中缺失和突变导致其表达降低,使p16抑癌功能丧失,造成宫颈上皮细胞异常增殖和分化失控,导致p16INK4A在子宫颈癌上皮组织全层表达。所以,p16INK4A作为一种免疫组织化学标志物应用于宫颈癌及其上皮内瘤变(CIN)的诊断有广阔的应用前景。

Objective To discuss the relationship between p16INK4A cervical carcinogenesis and the relationship and clinicopathological parameters.Methods P16INK4A were detected by immunohistochemistry in 40 cases of cervical squamous cell carcinoma,30 cases of cervical intraepithelial lesion（CIN）,25 cases of.cervical cancer and adjacent normal cervical tissue expression.Results The expression rate of p16INK4A in cervical intraepithelial lesions was 86.6%.The positive rate of cervical squamous cell carcinoma showed the expression of 100%.Normal cervical tissue were negative.Conclusion The deletion and mutation of p16 gene in cervical carcinoma led to reduced expression.lossing of p16 tumor suppressor function resulted in cervical epithelial cell proliferation and differentiation out of control and led to p16INK4A in cervical epithelium expression of full thickness.P16INK4A immunohistochemistry as a marker of diagnosis of cervical carcinoma and intraepithelial lesions（CIN）is very promising.