固相萃取(SPE)和衍生化条件的对GC/MS及LC/MS/MS法检测莱克多巴胺灵敏度的影响(英文)

Effects of SPE and Derivatization Conditions on the Sensitivities of GC/MS and LC/MS/MS in the Detection of Multi-functional Ractopamine

本实验探讨影响GC/MS以及LC/MS/MS检测灵敏度的影响因素。对GC/MS法的固相萃取(SPE)和衍生化条件进行优化,测试了SPE、加热时间、温度、溶液及碱基化等因素。同时测试这些条件对LC/MC/MC法的灵敏度。结果表明,碱基化为影响GC/MS法灵敏度的主要因素,能提高灵敏度,对衍生化条件也有影响。通过对样本进行固相萃取,碱基化处理,GC/MS法的灵敏度为0.5ng/mL,LC/MC/MC法的为0.25ng/mL。采用这种更灵敏的方法,尿液中莱克多巴胺的测试窗口期可由2d提高到6d。经过优化的GC/MS法及LC/MS/MS方法灵敏度更高,适合猪尿中莱克多巴胺的检测。

To get a high-sensitivity method for detection of ractopamine in urine samples of pigs,SPE and derivatiztion conditions were optimized.Among the examined factors including SPE,heating time,temperature,solution and neutralization,neutralization was found as the most important to GC/MS sensitivity,which affected the derivatization conditions and improved the sensitivity.The sensitivities of GC/MS and LC/MC/MC were 0.5 ng/mL and 0.25 ng/mL,respectively.By this higher sensitivity method,the detection window of ractopamine in urine can be improved from 2 days up to 6 days.