惠州地区客家人牙周病致病菌PCR分析

Identification of periodontopathogens in periodontitis patients on Hakka in Huizhou by PCR

目的应用聚合酶链式反应分别检测22例惠州地区客家牙周病患者及110例牙周健康人龈下菌斑中8种牙周病相关致病菌类型,分析不同的细菌类型与慢性牙周炎的相关性,为该地区牙周病的防治提供细菌学资料。方法取健康人和牙周炎患者龈下菌斑,水煮法提取DNA,采用8种常见牙周病可疑致病菌的特异引物,聚合酶链式反应(PCR)扩增16SrDNA片段来确定细菌种类。SPSS统计分析牙周炎与各种细菌相关性及与健康人组的差异显著性。结果惠州地区客家人慢性牙周病组除黄褐二氧化碳噬纤维菌(Co)外,其他细菌的检出率均显著高于健康组,差异有统计学意义(P<0.05)。结论惠州地区客家人的牙周病相关致病菌同国内其他地区相比有自己的分布特点。细菌与牙周炎的发病相关,健康体检及临床牙周炎的患者可结合细菌学检查进行诊断、预防和治疗。

Objective To establishthe relationship between periodontopathogens and periodontitis in Huizhou. Methods Subginggival plague samples were collected from 22 chronic periodontitis patients and 110 healthy adults. DNA from all samples were extracted by boiled methods,and 8 types of periodontophathogens in periodontitis patients were identified by amplifying the 16S rDNA with polymerase chains reaction （PCR）. The results were analyzed by SPSS. Results Except for Co,the percentages of detection rate for other periodontopathogens in periodontitis were higher than the control group （P〈0.05）. Conclusion The types of periodontopathogens of periodontitis patients showed their difference with other regions,which may be helpful for diagnosis, prevention and treatment of periodontitis in this area.