松杉灵芝提取物体外抗氧化活性的研究

Evaluation of the Antioxidant Activity of Extracts from Ganoderma tsugae in Vitro

利用1,1-二苯基-2-苦基肼自由基(.DPPH)、脂质过氧化(LPO)和羟基自由基(.OH)3种抗氧化活性分析方法,对松杉灵芝石油醚提取物(PE)、氯仿提取物(TE)、甲醇提取物(ME)、水提取物(WE)进行抗氧化评价。结果表明,在2 mg.mL-1时,各组提取液都具有明显的抗氧化活性,对.DPPH自由基的清除率分别为68.73%(ME)、65.28%(WE)、57.78%(PE)和51.56%(TE);对脂质过氧化的抑制率分别为64.55%(ME)、59.72%(WE)、55.63%(PE)和49.31%(TE);对.OH自由基的清除率分别为100.00%(WE)、81.26%(ME)、75.07%(TE)和60.34%(PE)。提取物浓度与抗氧化性成正相关,甲醇层和水层的抗氧化活性最好。

In this paper,using the analysis methods of 1,1-diphenyl-2-picrylhydrazyl radical（.DPPH）,lipid peroxidation and hydroxyl radical（.OH）,the antioxidant activity of extracts of petroleum（PE）,chloroform（TE）,methanol（ME） and water（WE） from Ganoderma tsugae was evaluated.The results showed all kind extracts had obvious antioxidant activity at 2 mg.mL-1.The scavenging rates of.DPPH were 68.73%（ME）,65.28%（WE）,57.78%（PE） and 51.56%（TE） respectively,the inhibition rates of lipid peroxidation were 64.55%（ME）,59.72%（WE）,55.63%（PE） and 49.31%（TE）,and the clearance rates of.OH were 100.00%（WE）,81.26%（ME）,75.07%（TE） and 60.34%（PE）.The concentration of the extracts was in direct proportion to the antioxidant activity,and methanol extracts and water extracts had the best antioxidant activity.