摘要
目的:探讨胃癌组织中RORγt mRNA的表达水平并观察其与临床病理组织分化程度的相关性。方法:收集59例胃癌患者胃癌组织和癌旁组织标本,采用实时荧光定量PCR的方法检测RORγt mRNA的表达水平。结果:胃癌组织RORγt表达水平为(1.26±0.42),明显高于癌旁组织(0.35±0.14),且与肿瘤组织分化程度相关,即RORγtmRNA表达水平越高,分化越低。结论:胃癌患者肿瘤微环境存在RORγt的高表达,表明RORγt可能参与了胃癌的免疫病理进程。
Objective： To investigate the expression of retinoid-related orphan receptor gammat（RORγt） mRNA in the patients with gastric cancers and to observe the relationship between RORγt mRNA and the clinicopathologic type.Methods： Realtime fluorescence quantitative method was utilized to detect the expression of RORγt mRNA in 59 specimens of gastric cancer tissues and adjacent cancer tissues.Results： The expression of RORγt in gastric cancer tissues were markedly higher than that in adjacent cancer tissues,and the higher expression of RORγt mRNA was obviously correlated with the clinicopathologic type.RORγt mRNA showed the higher value in poorly differentiated clinicopathologic type group than in well differentiated group.Conclusion： There was higher expression of RORγt in tumor microenvironment of gastric cancer patients,which indicated RORγt might be involved in the immunopathogenesis of gastric cancer.
出处
《江苏大学学报（医学版）》
CAS
2010年第6期473-475,480,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏省卫生厅面上科研项目(H200859)
镇江市社会发展项目(SH2008042)
