电视胸腔镜辅助下建立猪心肌梗死模型

Establishment of myocardial infarction model by video-assisted thoracoscopy in swine

目的:介绍一种新的心肌梗死模型制作方法,为心肌梗死的中长期研究及完成一些疑难的、需要反复进胸的心肌梗死实验提供良好的平台。方法:取8～11个月龄健康家猪,体质量30 kg左右,氯胺酮、吗啡麻醉,气管插管,呼吸气囊辅助呼吸给氧。取右侧斜卧位,胸骨左缘第3肋间切口长约3.0 cm,于上一肋间腋前线处取一约1.5 cm切口置光源。进胸后在电视胸腔镜辅助下显露钝缘支,5-0 prolene无创缝扎,术毕鼓肺排气拔除胸引管,同时关胸。主要观察指标:心肌酶谱、肌钙蛋白、心电图,同时标本进行HE染色。结果:该方法可以完成急性、亚急性、慢性心肌梗死模型建立,结果可靠,重复性好,模型猪能长期存活,生存率高,术后易于长期饲养。结论:在电视胸腔镜辅助下能更好地完成猪心肌梗死模型。

Objective： To introduce a new method to establish myocardial infarction model,which provided a good platform for the long-term study of myocardial infarction and met the need to ingress into the chest repeatedly.Methods： Experimental pigs were 8-11 month-old healthy ones,weighed about 30 kg,anesthetized with ketamine,morphine,and trachea intubated,oxygen balloon-assisted breathed.Taken sternum left edge third intercostal incision of about 3.0 cm on the right oblique position and an other incision about 1.5 cm in the anterior axillary line at the up intercostal to go through light source.Entered the pleural and exposed the blunt marginal branch by video-assisted thoracoscopy,and sutured with 5-0 prolene.Inflated the lung after the operation,and extracted chest tubes.At the same time closed chest.Main outcome measures： myocardial enzymes,cardiac troponin,ECG,HE staining specimens.Results： This method can be used to establish acute,subacute,chronic myocardial infarction model,the results are reliable,reproducible,and to be the long-term survival of pig model with a high survival rate and easy to keep long-term feeding.Conclusion： Myocardial infarction model can be established better by video-assisted thoracoscopy.