卵巢癌细胞系中的ApoaⅡ剪切变异体蛋白表达与顺铂治疗关系的体外实验研究

In Vitro Study on the Relationship between the Expression Of Apoa Ⅱ Shear Variants in Ovarian Carcinoma Cell Lines and Cisplatin Treatment

目的:观察不同浓度顺铂处理前后卵巢癌细胞中ApoaⅡ剪切变异体蛋白表达情况。方法:用不同浓度顺铂处理卵巢上皮癌细胞系A2780和SKOV3,采用RT-PCR和免疫细胞化学方法动态观察顺铂体外处理卵巢癌细胞后ApoaⅡ剪切变异体mRNA及蛋白表达情况。结果:顺铂体外处理卵巢癌铂类敏感细胞前后均未见ApoaⅡ剪切变异体mRNA表达,但ApoaⅡ剪切变异体蛋白随顺铂作用强度及时间的增强而表达量增加。结论:卵巢上皮癌细胞系中ApoaⅡ剪切变异体蛋白表达与顺铂作用相关。

Objective： To investigate the relationship between the expressions of Apoa Ⅱ shear variant in ovarian cancer cells and the different concentrations of cisplatin treatment. Methods： RT-PCR and immunocytochemistry were used to detect the expression of Apoa Ⅱ shear variant mRNA and protein in ovarian cancer cells with different concentrations of cisplatin treatment in vitro. Results： There was no expression of Apoa Ⅱ shear variant mRNA in ovarian cancer cells with or without cisplatin treatment In vitro, but the expression of Apoa Ⅱ shear variant protein increased with the intensifying of the effect of cisplatin and the expanding of time. Conclusion ： The expression of Apoa Ⅱ shear variants protein in ovarian cancer cell lines is associated with the effect of cisplatin in vitro.