摘要
用0．1mol/L氯化钠、0．02mol/L,pH7．4的磷酸缓冲液和蒸馏水，于40℃和4℃对浙江酶、华芬酶和Avizyme－1500酶制剂分别抽提1．5小时和12小时，对3种酶制剂中的果胶酶活力进行测定。结果表明：用0．1mol／L氯化钠于4℃抽提12小时，对浙江酶制剂中的果胶欧抽提效果最好；用蒸馏水于4℃抽提12小时，对华芬酶和Avizyme－1500酶制剂中的果胶酶抽提效果最好。
Determined activity of pectinase extracted from Zhejiang, Huafen and Avizyme- 1500 crudeenzyme preparation at 4℃ and 40℃ for 1.5h and 12h by 0.1mol/L NaCl, 0.02mol/L sodium phosphatebuffer, pH7 .4 and distilled water. The results showed that activity of pectinase extracted by 0.1mol/LNaCl from Zhejiang crude enzyme preparation at 4℃ for 12h is higher than the others, but huafen andAvizyme- 1500 crued enzyme preparation extracted by distilled water at 4℃ for 12h is the highest.
出处
《兽药与饲料添加剂》
1999年第3期8-9,共2页
Veterinary Pharmaceuticals & Feed Additives
