摘要
对从土壤中筛选到的菌株ＤＧＣ－００７进行ＵＶ诱变，选育到菌株ＤＧＣ－０４８．对影响突变株产酶的条件进行了优化，初步确定了ＤＧＣ－０４８合适的产酶摇瓶培养基组成．在发酵温度为３０℃，培养基起始ｐＨ７．５～８．０的条件下，接种量１０％，发酵４８ｈ，产酶可达３５０μｍｏｌ／（ｍｉｎ·Ｌ）
A extracellular cholesterol oxidase producing bacteriun strain(DGC007) was isolated from soil and mutated through UV light radiation.The mutant DGC48 was obtained.The effects of fermentation conditions on cholesterol oxidase production were studied and opotimized.The enzyme yield of 350U/L was obtained when the mutant was cultured under the following conditions:tempenature,30 ;initial pH 7.5￣8;inoculum size,10%;fermentation period, 48 h.
基金
国家轻工业局轻工科技发展计划项目
