摘要
研究了假单胞菌ＪＷ１２脂肪酶在２Ｌ和２５Ｌ容积发酵罐的补料分批发酵工艺．通过调整碳源补加速率，控制产酶期发酵液ＰＨ在８．２左右，能有效提高脂肪酶的酶活和表观生产率．在２５Ｌ标准发酵罐中，连续补加吐温－８０，最高脂肪酶酶活为１２９．２μｍｏｌ／（ｍｉｎ·ｍＬ），表观生产率为１５．
Production of Pseudomonas sp.JW12 lipase by fedbatch culture was studied in 2 L and 25 L fermentors.When we controlled supernante pH value at 8.2 or so by adjusting carbon source feeding velocity,lipase activity and productivity app were increased effectively.Lipase of 129.2 mol/(minmL),productivity app of 15.91 were obtained by feeding Tween 80 culure in a 25 L fermentor.
基金
国家"九五"攻关项目
