高效液相色谱法测定马铃薯中α─茄碱含量

应用乙腈-磷酸二氢钾（75:25，V／V）为流动相，在YWG－C18反相柱上采用外标法测定了马铃薯中不同部位上α─茄碱含量，方法快速、简便，重现性好。α─茄碱检测线性范围为0．1～1．0mg／ml，r＝0．9994，平均回收率为91．5％，变异系数为3．84％。

A simple.rapid and accurate quantitative method was established for the determination of α-solanine inpotato by High-performance in potato were extracted with TFA-H2O-CH3CN-HACI50:30:20: 1,(V/V)]solution.Separation was obtained by using YWG C18 column (4.6mmi.d.x25mm,5 μ m) and mobile phase ofacctomitrile-0.02mol/L potassium dihydrogen phosphate (75:25.V/V) with fowl rate of 0.7ml/min.The eluates weremonitored with UV/VIS detector at 208nm.The retention time for α-solanine was 4.27min.The calibration curve waslinear in the range of 0.05～1.00mg/ml with r=0.9994.The average recovery tar α-solanine was 91 .5% with acoefficient of variation of 3 .84%.The content of α-solanine in potato from different areas was also determined.