海洛因依赖者血清中Th1和Th2细胞产生主要细胞因子水平的研究

A study on the level of cytokines produced mainly by Th1/Th2 cells in sera from heroin addicts

目的:通过检测Th1、Th2细胞产生主要细胞因子IFN-γ、IL-4在海洛因依赖者血清中水平的变化,为充分认识海洛因对机体免疫功能的影响提供依据。方法:93例海洛因依赖者按吸食海洛因时间长短分为三组(2年以内、5～10年、10年以上),31例正常人血清作为对照组,用双抗体夹心酶联免疫吸附试验(ELISA)检测各组人血清IFN-γ、IL-4水平。结果:各组海洛因依赖者血清细胞因子IFN-γ水平高于正常对照组(P<0.05),而IL-4水平低于正常对照组(P<0.05)。吸食海洛因2年到5-10年到10年以上,IFN-γ水平呈先高后低变化(108.52±25.84pg/ml、133.13±36.35pg/ml、113.4±38.17pg/ml),IL-4水平呈先低后高变化(129.18±83.42pg/ml、76.35±47.01pg/ml、139.74±111.37pg/ml)。结论:Th1、Th2细胞产生主要细胞因子IFN-γ、IL-4在海洛因依赖者血清中水平改变明显,证明海洛因依赖者存在Th1/Th2功能紊乱。特别是吸食5～10年间以Th1亢进,Th2降低为主。

Objective：To discuss the alteration of the levels of cytokines IFN-γ,IL-4 produced mainly by Th1/Th2 cell in the sera of the heroin addicts in order to provide evidences for understanding the affection by heroin on the immune function.Methods：93 cases of heroin addicts were equally divided into 3 groups.As those who employed to detect their sera levels of IFN-γ,IL-4 of thein sera were detected.31 cases of normal human sera were used as controls.Results：The levels of cytokine IFN-γ in the sera of heroin addicts grouped by various periods of time were higher than those of the normal control group（P〈0.05）,while the levels of IL-4 were lower compared to the normal control group（P〈0.05）.The levels of IFN-γ was increasing in groups of within 10 years,yet decreasing in those of more than 10 years（108.52±25.84 pg/ml for groups of within 2 years,133.13±36.35 pg/ml for groups of 5～10 years,and 113.4±38.17 pg/ml for groups of more than 10 years,respectively）,while IL-4 levels showed the opposite-decreased for groups of within10 years,yet increased for groups of more than 10 years（129.18±83.42 pg/ml,76.35±47.01 pg/ml,139.74±111.37 pg/ml）.Conclusion：The levels of cytokines of IFN-γ and IL-4 produced mainly by Th1/Th2 cell in the sera from the heroin addicts change significantly.The Th1/Th2 functional imbalance presents in heroin addicts showing up-regulation of Th1 cell function but down-regulation of Th2 cell function,especially during the period of 5～10 years.