铁线莲‘Multi-Blue’体细胞胚诱导和植株再生

Embryogenesis and plant regeneration of Clematis 'Multi-Blue'

以铁线莲品种‘Multi-Blue’的茎尖为外植体,1/2MS为基本培养基,进行胚性愈伤组织和体细胞胚的诱导及植株再生条件的选择。结果表明:茎尖外植体在1/2MS+TDZ(1.0 mg/L)+NAA(0.5 mg/L)+2%蔗糖+0.7%琼脂的固体培养基中培养时可形成淡黄色块状至颗粒状愈伤组织,经继代培养后可逐渐形成胚性愈伤组织;体细胞胚诱导以1/2MS+TDZ(0.2 mg/L)+IBA(0.1 mg/L)+ABA(0.3 mg/L)+CH(0.5 g/L)为佳,诱导率可达96%;而适宜的体细胞胚萌发培养基为1/2MS+6-BA(0.2 mg/L)+IBA(0.05 mg/L)。体胚再生植株经驯化后,移栽成活率可达80%以上。

Using stem tip of Clematis ＇Multi-Blue＇ as explants and 1/2 MS as culture media,the suitable conditions for induction of embryogenic callus and somatic embryos,as well as plant regeneration,were determined.The results indicated that the optimal conditions for induction of embryogenic callus were 1/2 MS supplemented with 1.0 mg/L TDZ,0.5 mg/L NAA and 2 % scourse plus 0.7 % agar.Explants cultured in this solid medium could form yellow massive or granular callus and produced embryogenic callus after subculture.Suitable media for somatic embryo induction were 1/2 MS added with 0.2 mg/L TDZ and 0.1 mg/L IBA plus 0.5 g/L CH,with an induction rate of 96%.The germination rate of somatic embryos was the highest when the plant growth regulators were 0.2 mg/L 6-BA and 0.05 mg/L IBA.The plantlets with root were successfully transplanted to the medium with vermiculite.The survival rate of regeneration plant could be up to at least 80 %.