大孔吸附树脂纯化江南卷柏总黄酮的工艺研究

Purification technology of total flavonoids from Selaginella moellendorfii Hieron with macroporous adsorption resin

目的:确定纯化江南卷柏总黄酮的大孔树脂及其工艺参数。方法:以总黄酮的含量为指标,从3种不同型号的大孔树脂中筛选纯化江南卷柏总黄酮的最佳树脂;以江南卷柏总黄酮的回收率为指标,通过单因素试验确定该树脂分离纯化的工艺条件。结果:D101型树脂对江南卷柏总黄酮有较好的吸附和解吸附性能;其纯化江南卷柏总黄酮的较佳工艺条件为:药材量与树脂量的比例为1∶3,以8BV(8倍体积)70%乙醇洗脱,流速为2BV·h-1,树脂每次可重复使用3次。在所确定的工艺条件下,江南卷柏总黄酮纯度可达50%以上,回收率可达85%以上。结论:该工艺条件可用于江南卷柏总黄酮的纯化。

Objective：To determine the optimum macroporous resin the parameters of technology purificating total flavonoids from Selaginella moellendorfii Hieron with the resin.Methods：With the content of total flavonoids from Selaginella moellendorfii Hieron as index,screen out the optimal resin which can purify the from 3 different resins,and determin the technology condition purifying the by single-factor experiments.Results：The total flavonoids from Selaginella moellendorfii on D101 macroporous resin showed the best effection of adsorption desorption.The preferable technology of purifying the total flavonoids was：the ratio of weight of the herbs and resin was 1∶3,eluted at a velocity of 2 BV·h^-1 by a volume of 8 BV·h^-1 of 70% alcohol.The resin could be reused for 3 times before treating.Under the technology the recovery rate of total flavonoids was more than 85% and the purity was more than 50%.Conclusion：The D101 macroporous resin is suitable to purify the total flavonoids from Selaginella moellendorfii Hieron.