反相高效液相色谱法同时测定黄术健胃片中野黄芩苷、盐酸小檗碱及丹皮酚的含量

RP-HPLC simultaneous determination of three kinds of components in Huangzhu Jianwei tablets

目的:建立同时测定黄术健胃片中野黄芩苷、盐酸小檗碱及丹皮酚的含量测定方法。方法:采用RP-HPLC法,Agilent Zorbax SB-C18色谱柱(250mm×4.6mm,5μm);以乙腈-20mmol·L-1乙酸铵溶液(用冰醋酸调pH至4.0)为流动相梯度洗脱;流速为1.0mL·min-1;检测波长为335nm。结果:野黄芩苷的线性范围为0.05～0.26μg,r=1.0000,平均回收率为102.2%;盐酸小檗碱的线性范围为0.32～1.62μg,r=0.9998,平均回收率为104.1%;丹皮酚的线性范围为0.32～1.62μg,r=0.9998,平均回收率为99.6%。结论:该方法简便、快速、准确,重现性好,可作为该制剂中野黄芩苷、盐酸小檗碱及丹皮酚的含量测定方法。

Objective：To establish a method for the determination of scutellarin,berberine hydrochloride and Paeonol simultaneously.Methods：The RP-HPLC system consisting of a Agilent Zorbax SB-C18 column （250 mm×4.6 mm,5 μm）, acetonitrile-20 mmol·L^-1 acetic ammonium（adjusted pH to 4.0 with acetic acid glacial） as mobile phase（using gradient） with flowing rate 1.0mL·min^-1 and detected at 335 nm.Results：The calibration curve of scutellarin was linear from 0.05-0.26 μg with r=1.0000, the average recovery was 102.2%; The calibration curve of berberine hydrochloride was linear from 0.32-1.62 μg with r=0.9998, the average recovery was 104.1%; The calibration curve of paeonol was linear from 0.32-1.62 μg with r=0.9998, the average recovery was 99.6%.Conclusion：The method is simple, quick, accurate and reproducible, and can be used for the quality control of the preparation all-around.